480026

NBD-Binding Peptide II, Cell Permeable, CTP-NBD

A cell-permeable cytoplasmic transduction peptide (CTP; YGRRARRRARR) fused to the NEMO binding domain peptide (NBD; TALDWSWLQTE) that acts as a potent inhibitor of TNFα-stimulated NF-κB activity (IC₅₀~ 50 µM in HCT116 cells).

• Synonyme(s) : NBD-Binding Peptide II, Cell Permeable, CTP-NBD, NEMO-Binding Domain Binding Peptide II, Cell-Permeable, CTP-NBD
• Formule empirique (notation de Hill): C₁₂₃H₁₉₇N₄₇O₃₂
• Poids moléculaire : 2846.18
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.77

Propriétés

• Niveau de qualité: 100
• Pureté: ≥95% (HPLC)
• Forme: solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated (hygroscopic); protect from light
• Couleur: white
• Solubilité: water: 50 mg/mL
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C

Description

Description générale

A cell-permeable cytoplasmic transduction peptide (CTP; YGRRARRRARR) fused to the NEMO binding domain peptide (NBD; TALDWSWLQTE) that acts as a potent inhibitor of TNFα-stimulated NF-κB activity (IC₅₀ ~ 50 µM in HCT116 cells). Acts by interfering with the formation of active IKK complex and thereby prevents IκBα phosphorylation and its subsequent proteasomal degradation. Also blocks LPS-mediated increase in inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) activities. Colon-targeted capsules containing CTP-NBD are shown to reduce 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis in rodent models (4.5 mg/kg/,p.o.) in vivo and diminish activities of myeloperoxidase and several inflammatory mediators. Antennapedia fused NBD cell-permeable peptide is also available (>Cat. No. 480025).

Actions biochimiques/physiologiques

Cell permeable: yes

Primary Target
NEMO-binding peptide

Reversible: yes

Conditionnement

Packaged under inert gas

Yes

Avertissement

Toxicity: Standard Handling (A)

Séquence

Ac-YGRRARRRARRTALDWSWLQTE-NH₂

Autres remarques

Hong, S., et al. 2012. Mol. Pharm.9, 1310.
Kim, D., et al. 2006. Exp. Cell Res.312, 1277.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable