427211

Lactate Dehydrogenase, Porcine Heart

• Synonyme(s) : Lactate Dehydrogenase, Porcine Heart
• Numéro CAS: 9001-60-9
• Numéro de classification (Commission des enzymes): 1.1.1.27 (BRENDA, IUBMB)
• Numéro MDL: MFCD00131466
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.54

Propriétés

• Forme: lyophilized
• Niveau de qualité: 100
• Activité spécifique: ≥300 units/mg protein
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated
• Impuretés: ≤0.01% PK, MK; ≤0.03% GPT, GOT; ≤0.1% MDH
• pl: 4.6
• Solubilité: aqueous buffer: soluble
• Conditions d'expédition: ambient
• Température de stockage: −20°C

Description

Description générale

Native lactate dehydrogenase from porcine heart. A tetrameric enzyme that catalyzes the reversible conversion of pyruvate to lactate. The overall equilibrium of this reaction favors lactate formation. Has an optimal pH of 7.0 and a pI of 4.6. Note: 1KU = 1000 units.

Native lactate dehydrogenase from porcine heart. A tetrameric enzyme that catalyzes the reversible conversion of pyruvate to lactate. The overall equilibrium of this reaction favors lactate formation. Note: 1KU = 1000 units.

Note: 1 KU = 1000 units

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit is defined as the amount of enzyme that will cause the oxidation of one micromole of NADH per minute at 25°C, pH 7.4

One unit is defined as the amount of enzyme that will oxidize 1.0 µmol NADH per min at 25°C, pH 7.4.

Reconstitution

Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 24 h at 4°C. Do not freeze solutions.

Autres remarques

Kornberg, A. 1995. Methods Enzymol.1, 441

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1