217356

Carboxypeptidase B, Porcine Pancreas

• Synonyme(s) : Carboxypeptidase B, Porcine Pancreas
• Numéro CAS: 9025-24-5
• Numéro de classification (Commission des enzymes): 3.4.17.2 (BRENDA, IUBMB)
• Numéro MDL: MFCD00081476
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: Porcine pancreas
• Niveau de qualité: 100
• Forme: lyophilized
• Activité spécifique: ≥100 units/mg protein
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• pl: 6.0
• Solubilité: water: 1 mg/mL
• Activité étrangère: Carboxypeptidase A <1.0%; Chymotrypsin, none detected; Trypsin ≤5%
• Conditions d'expédition: ambient
• Température de stockage: −20°C
• InChI: 1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)
• Clé InChI: TWURVFFNODFJBJ-UHFFFAOYSA-N

Description

Description générale

Native carboxypeptidase B catalyzes the release of C-terminal from basic amino acids, arginine, lysine, or ornithine from polypeptides. Inhibited by EDTA and other Zn²⁺ chelators.

Native carboxypeptidase B catalyzes the release of C-terminal from basic amino acids, arginine, lysine, or ornithine from polypeptides. Inhibited by EDTA and other Zn²⁺ chelators. Has an optimal pH of 7.0-9.0 and a pI of 6.0.

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Hip-Arg per min at 25°C, pH 7.7.

Forme physique

Lyophilized from 360 µM HEPES, 1.41 µM zinc chloride, 1.41 mg/ml Trehalose, pH 7.4.

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Autres remarques

Folk, J.E. 1970. Methods Enzymol.19, 504.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS08,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H315,H319,H334,H335
• Conseils de prudence: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable