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Key Documents

126579

Sigma-Aldrich

Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free

Synonyme(s) :

Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free

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About This Item

Numéro CAS:
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.25

Pureté

≥95% (Albumin, electrophoresis)

Forme

lyophilized powder

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze

Impuretés

≤0.2% fatty acids
≤2.0% Ash
≤5% moisture
≤50 EU/mg endotoxins

Couleur

pale green

Solubilité

aqueous buffer: 20 mg/mL
water: 20 mg/mL

Conditions d'expédition

ambient

Température de stockage

2-8°C

Description générale

Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free is designed for use in cell culture systems and animal perfusion studies. It has a pH of 7.0 (1% solution).
Bovine serum albumin (BSA), a member of the serum albumin family, contains three domains and two sub-domains under each. It is a non-glycosylated globular, α-helical protein that has 17-disulfide bonds.

Application

Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free has been used in the preparation of advanced glycation end-products (AGEs). It has also been used to dilute lipopolysaccharide (LPS) and tumor necrosis factor (TNF) and in the stimulation and fixation of whole blood cells.

Actions biochimiques/physiologiques

Bovine Serum Albumin (BSA) is the most important constituent of cell culture media. It aids embryonic stem cells (hESC) differentiation. BSA facilitates the transport of drugs, hormones, and fatty acids. It elicits high solubility and is suitable in the cosmetic and pharmaceutical industries. It is applicable as a blocking agent in enzyme-linked immunosorbent assay (ELISA).

Avertissement

Toxicity: Standard Handling (A)

Notes préparatoires

Material may take up to 24 h to fully dissolve.

Reconstitution

Following reconstitution, store in the refrigerator (4°C). 0.1% NaN₃ may be added as a preservative. Stock solutions are stable for up to 3 months at 4°C.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Dan Ye et al.
Structure (London, England : 1993), 26(11), 1513-1521 (2018-09-18)
We introduce resonant soft X-ray scattering (RSoXS) as an approach to study the structure of proteins and other biological molecules in solution. Scattering contrast calculations suggest that RSoXS has comparable or even higher sensitivity than hard X-ray scattering because of
Xiao-Yan Zhao et al.
Nature communications, 11(1), 2992-2992 (2020-06-14)
Activated protein C (APC) is a plasma serine protease with antithrombotic and cytoprotective functions. Based on the hypothesis that specific inhibition of APC's anticoagulant but not its cytoprotective activity can be beneficial for hemophilia therapy, 2 types of inhibitory monoclonal
Anthony Dobi et al.
Microvascular research, 133, 104098-104098 (2020-10-20)
During diabetes mellitus, advanced glycation end-products (AGEs) are major contributors to the development of alterations in cerebral capillaries, leading to the disruption of the blood-brain barrier (BBB). Consequently, this is often associated with an amplified oxidative stress response in microvascular
Ravi Kumar Murumalla et al.
Lipids in health and disease, 11, 175-175 (2012-12-25)
On the basis that high fat diet induces inflammation in adipose tissue, we wanted to test the effect of dietary saturated and polysunsaturated fatty acids on human adipose tissue and adipocytes inflammation. Moreover we wanted to determine if TLR2 and
Michael A Kalwat et al.
ACS chemical biology, 11(4), 1128-1136 (2016-02-02)
Novel strategies are needed to modulate β-cell differentiation and function as potential β-cell replacement or restorative therapies for diabetes. We previously demonstrated that small molecules based on the isoxazole scaffold drive neuroendocrine phenotypes. The nature of the effects of isoxazole

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