06-413

Anti-NFκB p52 Antibody

Upstate^®, from rabbit

• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: using Protein A
• Espèces réactives: human
• Fabricant/nom de marque: Upstate^®
• Technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG
• Numéro d'accès NCBI: NM_019408
• Numéro d'accès UniProt: Q00653
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... NFKB1(4790)

Description

Description générale

The NFκB transcription factor was originally identified as a protein complex consisting of a 65 kDa DNA binding subunit and an associated 50 kDa protein. The 65 kDa subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino terminus of a precursor designated p105. A cDNA has been isolated that encodes an alternative DNA binding subunit of NFκB. It is synthesized as a 100 kDa protein that is expressed in a variety of cell types and, like p105, undergoes cleavage to generate its NFκB subunit, in this case a protein designated p52 (previously referred to as p49). In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells.

Spécificité

Recognizes NFκB p52 and its precursor.

Immunogène

KLH conjugated synthetic peptide (CYNPGLDGIIEYDDFK) contained within amino acids 4-19 of human NF κB, p52

Application

Detect NFκB p52 with Anti-NFκB p52 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in IP & WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Qualité

routinely evaluated by immunoblot on RIPA lysates from Raji cells

Description de la cible

52 kDa, and precursor (100 kDa)

Forme physique

Format: Purified

Protein A Purified immunoglobulin in in 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% NaN3 as a preservative.

Unpurified

Stockage et stabilité

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Remarque sur l'analyse

Control
Raji cell lysate, Jurkat whole cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable