05-443
Anti-VEGF Antibody, clone JH
clone JH, Upstate®, from mouse
Synonyme(s) :
Vascular permeability factor, vascular endothelial growth factor, vascular endothelial growth factor A, vascular endothelial growth factor isoform VEGF165
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
100
400
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
JH, monoclonal
Espèces réactives
rat, mouse, rabbit, human
Fabricant/nom de marque
Upstate®
Technique(s)
immunohistochemistry: suitable (paraffin)
Isotype
IgG1κ
Adéquation
not suitable for Western blot
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... VEGFA(7422)
Description générale
The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines. Flt3 is also expressed the placenta, testis, spleen, thymus, bone marrow, brain and in cerebellar Purkinje cells. The VEGF receptors stimulate the Ras-MAP kinase pathway, suggesting that it signals as a conventional receptor tyrosine kinase.
Spécificité
Other species not tested.
Recognizes 34-54 kDa isoforms of Vascular Endothelial Growth Factor/Vascular Permeability Factor (VEGF/VPF).
Immunogène
Recombinant human VEGF121. Clone JH121
Application
Immunohistochemistry: This lot of antibody detected VEGF in formalin fixed, paraffin embedded, human.tissues, using a 1:50 dilution incubated overnight at 4°C.
Immunoprecipitation: 2 µg of this antibody has been reported to immunoprecipitate VEGF from 1 mg of protein lysate.
Immunoprecipitation: 2 µg of this antibody has been reported to immunoprecipitate VEGF from 1 mg of protein lysate.
Research Category
Signaling
Signaling
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
This Anti-VEGF Antibody, clone JH is validated for use in IH(P) for the detection of VEGF.
Qualité
Biological Blockade: Incubation of HUVEC cells with 5 µg/mL of a previous lot and 5 ng/mL of VEGF (Catalog # 01-185) has shown growth inhibition of 20-70%.
Description de la cible
34-54 kDa isoforms
Forme physique
Format: Purified
Protein G Chromatography
Purified mouse monoclonal IgG1k in buffer containing 10 mM PBS, pH 7.4.
Stockage et stabilité
Stable for 1 year at from date of receipt.
Remarque sur l'analyse
Control
Expressed in most tissue, including Pancreas, Kidney, Adenocarcinoma.
Expressed in most tissue, including Pancreas, Kidney, Adenocarcinoma.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
M Wartenberg et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 15(6), 995-1005 (2001-04-09)
Tumor vascularization is the rate-limiting step for the progression of cancer. Differential steps of tumor-induced angiogenesis were studied by a novel in vitro confrontation culture of avascular multicellular prostate tumor spheroids and embryoid bodies grown from pluripotent embryonic stem (ES)
C O Tibesku et al.
The open orthopaedics journal, 5, 177-180 (2011-06-03)
To evaluate the expression of VEGF by chondrocytes of hyaline cartilage during the course of osteoarthritis (OA). In 12 white New Zealand rabbits the anterior cruciate ligament (ACL) was resected to create an anterior instability of the knee. In 12
VEGF121, a vascular endothelial growth factor (VEGF) isoform lacking heparin binding ability, requires cell-surface heparan sulfates for efficient binding to the VEGF receptors of human melanoma cells.
Cohen, T, et al.
The Journal of Biological Chemistry, 270, 11322-11326 (1995)
Hiroshi Kubo et al.
Genes to cells : devoted to molecular & cellular mechanisms, 14(3), 407-424 (2009-02-21)
Although ex vivo expanded mesenchymal stem cells (MSC) have been used in numerous studies, the molecular signature and in vivo distribution status of MSC remain unknown. To address this matter, we identified numerous human MSC-characteristic genes--including nine transcription factor genes--using
Treatment-induced neoangiogenesis in cerebral arteriovenous malformations.
U Sure, N Butz, A M Siegel, H D Mennel, S Bien, H Bertalanffy
Clinical Neurology and Neurosurgery null
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