201677

Janus Green B

certified by the Biological Stain Commission, Dye content 65 %

• Synonyme(s) : 3-Diethylamino-7-(4-dimethylaminophenylazo)-5-phenylphenazinium chloride, Diazin Green S, Union Green B
• Formule empirique (notation de Hill): C₃₀H₃₁ClN₆
• Numéro CAS: 2869-83-2
• Poids moléculaire : 511.06
• Numéro C.I. (Colour Index): 11050
• Numéro CE : 220-695-6
• Numéro MDL: MFCD00011758
• Code UNSPSC : 12171500
• ID de substance PubChem : 24852018
• Nomenclature NACRES : NA.47

Propriétés

• Qualité: certified by the Biological Stain Commission
• Forme: powder
• Composition: Dye content, 65%
• Couleur: black; very dark brown; very dark green
• λmax: 395 nm; 660 nm (2nd)
• ε (coefficient d'extinction): ≥28000 at 651-677 nm in ethanol: water (1:1) at 0.01 g/L
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: 2-8°C
• Chaîne SMILES: [Cl-].CCN(CC)c1ccc2nc3ccc(cc3[n+](-c4ccccc4)c2c1)\N=N\c5ccc(cc5)N(C)C
• InChI: 1S/C30H31N6.ClH/c1-5-35(6-2)26-17-19-28-30(21-26)36(25-10-8-7-9-11-25)29-20-23(14-18-27(29)31-28)33-32-22-12-15-24(16-13-22)34(3)4;/h7-21H,5-6H2,1-4H3;1H/q+1;/p-1
• Clé InChI: XXACTDWGHQXLGW-UHFFFAOYSA-M

Description

Application

Janus Green B has been used in staining technique to determine cell density, to detect the presence of telocytes in trachea and lungs and used as a dye to visualize bonghan ducts inside lymphatic vessels of rabbits.

Actions biochimiques/physiologiques

Janus Green B belongs to the phenazine group of dyes. It is a dark green/dark brown/dark black powder. Janus Green B should be oxidized to become colored. Thus, this dye can be used to specifically stain mitochondria in living cells. It has been used to stain biomolecules, nucleic acids and chromosomes. In addition, it is also used to stain various living organisms including fungi, yeast cell, brain, spinal cord, sperms and tissue culture monolayer. Janus Green B acts as an antimalarial agent. It also facilitates diagnostic assays and diagnosis of disease related to amyloid accumulation.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)