HPLC Analysis of Butorphanol Enantiomers on Astec® CHIROBIOTIC® T

CONDITIONS
column
CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)
mobile phase
0.1 wt% ammonium formate in methanol
flow rate
1 mL/min
column temp.
25 °C
detector
UV, 254 nm
injection
1 μL
sample
1 mg/mL in methanol
Description
Legal Information
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC