- A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli.
A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli.
Molecular bioSystems (2010-03-20)
Ying Huang, William K Russell, Wei Wan, Pei-Jing Pai, David H Russell, Wenshe Liu
PMID20237646
ABSTRACT
By overexpressing the C-terminal domain of the ribosomal protein L11 to decrease release factor 1-mediated termination of protein translation, enhanced amber suppression is achieved in E. coli. This enhanced amber suppression efficiency allows the genetic incorporation of three N(epsilon)-acetyl-l-lysines into one GFP(UV) protein in E. coli.
MATERIALS