1.00213

NutriSelect^® prime Sorbitol MacConkey (SMAC) Agar

acc. FDA-BAM, ISO 16654

• Synonym(s): CT-SMAC Agar, MacConkey Agar with Sorbitol, SMAC Agar
• UNSPSC Code: 41106213
• NACRES: NA.74

Properties

• Agency: FDA-BAM
• Quality Level: 200
• form: powder
• shelf life: 5 yr
• composition:
  • Enzymatic digest of casein 17.0 g/L
  • Enzymatic digest of animal tissue 3.0 g/L
  • Sodium chloride 5.0 g/L
  • Bile salts No. 3 1.5 g/L
  • Sorbitol 10.0 g/L
  • Neutral red 0.03 g/L
  • Crystal violet 0.001 g/L
  • Agar-agar 15.0 g/L
• packaging: container of 500 g
• manufacturer/tradename: GranuCult^®
• technique(s): microbiological culture: suitable
• pH: 7.1±0.2 (25 °C)
• solubility: water: soluble
• application(s): food and beverages; pharmaceutical
• compatibility: for use with CT supplement (77981)
• shipped in: ambient
• storage temp.: 15-25°C
• suitability: selective and differential for Escherichia 0157:H7

Description

General description

GranuCult^® prime Sorbitol MacConkey (SMAC) Agar is a selective agar medium to isolate and differentiate enterohemorrhagic (EHEC) E. coli 0157 from food, animal feed and other samples.
The presence of bile salts and crystal violet inhibits the growth of gram-positive microbes thereby enhancing the growth of gram-negative microorganisms.
The specific selectivity of the medium is done by the addition of Cefixime potassium Tellurite supplement towards E. coli 0157:H7 and in turn suppresses the accompanying flora. Addition of sorbitol in the medium along with pH indicator neutral red is used to detect the sorbitol positive colonies which appear red in color. Typical colonies of (sorbitol negative) E. coli 0157 are transparent, colorless to pale yellowish with small diameter.
Enzymatic digest of casein and animal tissue provides nitrogen, vitamins, and amino acids to the growing microbes. Agar is the solidifying agent.

Application

GranuCult^® prime Sorbitol MacConkey (SMAC) Agar is used for the isolation and differentiation of sorbitol negative Escherichia coli serotype O157 from food, animal feed and other test samples.

Analysis Note

• The dehydrated media is granulated with light pink color.
• Typical colonies of (sorbitol negative) E. coli 0157 are transparent, colorless to pale yellowish with small diameter.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3