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Sigma-Aldrich

Anti-His Tag Antibody

clone 4D11, mouse monoclonal, Upstate

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43

product name

Anti-Histidine Tagged Antibody, clone 4D11, clone 4D11, Upstate®, from mouse

biological source

mouse

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

4D11, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG2aκ

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... HRG(3273)

Specificity

Recognizes tag sequence HHHHHHGS on either the N- or C-terminus of recombinant proteins; GS amino acids are required for recognition of 6His tag

Immunogen

N-terminal His-tagged and truncated TRAP protein corresponding to amino acids 26-248 from P. falciparum expressed in E coli.

Application

This Anti-Histidine Tagged Antibody (anti His antibody) clone 4D11 is validated for use in IC, WB for the detection of Histidine Tagged.

Quality

routinely evaluated by immunoblot on recombinant N-terminal and C-terminal His-tagged proteins

Target description

The apparent molecular weight is related to the tagged protein.

Physical form

Format: Purified
Protein G Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.014M phosphate buffer, pH 7.6, 0.175M NaCl, and 0.07% sodium azide.

Analysis Note

Control
Cells expressing the His-tag

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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K J Robson et al.
The EMBO journal, 14(16), 3883-3894 (1995-08-15)
Plasmodium sporozoites collected from oocysts, haemocoel and salivary glands of the mosquito show profound differences in their biological properties such as motility, ability to induce protective immune response and infectivity for vertebrate host cells. Sporozoites from salivary glands are much
Lisa A Mitchell et al.
Molecular human reproduction, 14(4), 235-243 (2008-02-05)
The process of capacitation is a pre-requisite for mammalian spermatozoa allowing them to gain the ability to fertilize an oocyte. A fundamental part of this mechanism is a dramatic increase in the level of tyrosine phosphorylation. Implicated in this process
Ruiping Zheng et al.
Journal of cell science, 123(Pt 21), 3734-3744 (2010-10-14)
In higher eukaryotic cells, long non-protein-coding RNAs (lncRNAs) have been implicated in a wide array of cellular functions. Cell- or tissue-specific expression of lncRNA genes encoded in the mammalian genome is thought to contribute to the complex gene networks needed
Wei-Hang Huang et al.
Heliyon, 9(11), e21486-e21486 (2023-11-29)
Originally extracted from Momordica charantia seeds, the antiviral and anti-tumor activities of Momordica anti-HIV protein MAP30 have become well known. Although MAP30 has been reported to possess antiviral activity against several human viruses, the current understanding of the MAP30-mediated antiviral
Francesca Mancini et al.
The EMBO journal, 28(13), 1926-1939 (2009-06-13)
MDM4 is a key regulator of p53, whose biological activities depend on both transcriptional activity and transcription-independent mitochondrial functions. MDM4 binds to p53 and blocks its transcriptional activity; however, the main cytoplasmic localization of MDM4 might also imply a regulation
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