Separation Options with Sephacryl^®

Five Sephacryl^® HR chromatography media are available as prepacked columns and in bulk packs (Table 1).

Table 1. Separation options with Sephacryl^® media
* For maximum resolution, apply as small sample volume as possible. Note that sample volumes less than 0.5% normally do not improve resolution.
† See Appendix 5 to convert linear flow (cm/h) to volumetric flow rates (ml/min) and vice versa.

Product	Fractionation range, Mr (globular proteins)	Fractionation range, Mr (dextrans)	Sample loading capacity*	Maximum pressure drop over the packed bed	Recommended operation flow†
HiPrep™ 16/60 Sephacryl® S-100 HR	1 × 103 to 1 × 105		≤ 5 mL	0.15 MPa, 1.5 bar,21 psi	0.5 mL/min
HiPrep™ 26/60Sephacryl® S-100 HR	1 × 103 to 1 × 105		≤ 13 mL	0.15 MPa, 1.5 bar,21 psi	1.3 mL/min
Sephacryl® S-100 HR(Bulk medium)	1 × 103 to 1 × 105		0.5% to 4% of total column volume	Column-dependent	10 to 35 cm/h
HiPrep™ 16/60Sephacryl® S-200 HR	5 × 103 to 2.5 × 105	1 × 103 to 8 × 104	≤ 5 mL	0.15 MPa, 1.5 bar,21 psi	0.5 mL/min
HiPrep™ 26/60Sephacryl® S-200 HR	5 × 103 to 2.5 × 105	1 × 103 to 8 × 104	≤ 13 mL	0.15 MPa, 1.5 bar,21 psi	1.3 mL/min
Sephacryl® S-200 HR (Bulk medium)	5 × 103 to 2.5 × 105	1 × 103 to 8 × 104	0.5% to 4% of total column volume	Column-dependent	10 to 35 cm/h
HiPrep™ 16/60Sephacryl® S-300 HR	1 × 104 to 1.5 × 106	2 × 103 to 4 × 105	≤ 5 mL	0.15 MPa, 1.5 bar,21 psi	0.5 mL/min
HiPrep™ 26/60Sephacryl® S-300 HR	1 × 104 to 1.5 × 106	2 × 103 to 4 × 105	≤ 13 mL	0.15 MPa, 1.5 bar,21 psi	1.3 mL/min
Sephacryl® S-300 HR(Bulk medium)	1 × 104 to 1.5 × 106	2 × 103 to 4 × 105	0.5% to 4% of total column volume	Column-dependent	10 to 35 cm/h
HiPrep™ 16/60Sephacryl® S-400 HR	2 × 104 to 8 × 106	1 × 104 to 2 × 106	≤ 5 mL	0.15 MPa, 1.5 bar,21 psi	0.5 mL/min
HiPrep™ 26/60Sephacryl® S-400 HR	2 × 104 to 8 × 106	1 × 104 to 2 × 106	≤ 13 mL	0.15 MPa, 1.5 bar,21 psi	1.3 mL/min
Sephacryl® S-400 HR(Bulk medium)	2 × 104 to 8 × 106	1 × 104 to 2 × 106	0.5% to 4% of total column volume	Column-dependent	10 to 35 cm/h
HiPrep™ 16/60Sephacryl® S-500 HR		4 × 104 to 2 × 107	≤ 5 mL	0.15 MPa, 1.5 bar,21 psi	0.5 mL/min
HiPrep™ 26/60Sephacryl® S-500 HR		4 × 104 to 2 × 107	≤ 13 mL	0.15 MPa, 1.5 bar,21 psi	1.3 mL/min
Sephacryl® S-500 HR(Bulk medium)		4 × 104 to 2 × 107	0.5% to 4% of total column volume	Column-dependent	10 to 35 cm/h
Sephacryl® S-1000 SF(Superfine,bulk medium)			0.5% to 4% of total column volume	not determined	2 to 30 cm/h