HPLC Analysis of Pilocarpine, Betaxolol, and Benzalkonium Chloride on Ascentis^® Express HILIC

column

Ascentis Express HILIC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)

mobile phase

[A] 20 mM ammonium acetate; [B] acetonitrile; (10:90, A:B)

flow rate

1 mL/min

column temp.

35 °C

detector

ESI (+), XIC m/z 304, 332 (BAC), 209 (pilocarpine), 308 (betaxolol)

injection

2 μL

sample

10 mg/L in acetonitrile