Accéder au contenu
MilliporeSigma

HPLC Analysis of Caffeine and Taurine on Ascentis® Express HILIC Column using Evaporative Light Scattering Detection (ELSD, Fast Analysis)

HPLC Analysis of Caffeine and Taurine on Ascentis® Express HILIC Column using Evaporative Light Scattering Detection (ELSD, Fast Analysis) application for HPLC

Matériaux

Colonne analytique

Réf. du produit
Description
Tarif

53967-U

Ascentis® Express HILIC HPLC Column

2.7 μm particle size, L × I.D. 5 cm × 3 mm

Étalon

Réf. du produit
Description
Tarif

T0625

Taurine

≥99%

56396

Caféine

certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

CONDITIONS

column

Ascentis Express HILIC, 5 cm x 3.0 mm I.D., 2.7 μm particles (53967-U)

mobile phase

[A] 100 mM ammoniumformate, pH 3.0 with formic acid; [B] acetonitrile; (15:85, A:B)

flow rate

0.6 mL/min

column temp.

25 °C

detector

ELSD, 55 °C, 3.5 bar nitrogen

injection

2 μL

sample

1000 μg/mL in 10:90, water: acetonitrile

Description

Remarque sur l'analyse

This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of caffeine and taurine. Elution is by aqueous normal phase (ANP) as an increase in organic will increase retention.

Informations légales

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS