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Oxidative stress and anti-oxidant enzyme activities in the trophocytes and fat cells of queen honeybees (Apis mellifera).

Rejuvenation research (2013-06-07)
Yu-Shan Hsieh, Chin-Yuan Hsu
RÉSUMÉ

Trophocytes and fat cells of queen honeybees have been used for delayed cellular senescence studies, but their oxidative stress and anti-oxidant enzyme activities with advancing age are unknown. In this study, we assayed reactive oxygen species (ROS) and anti-oxidant enzymes in the trophocytes and fat cells of young and old queens. Young queens had lower ROS levels, lower superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities, and higher thioredoxin reductase (TR) activity compared to old queens. These results show that oxidative stress and anti-oxidant enzyme activities in trophocytes and fat cells increase with advancing age in queens and suggest that an increase in oxidative stress and a consequent increase in stress defense mechanisms are associated with the longevity of queen honeybees.

MATÉRIAUX
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Description du produit

Roche
Essai de longueur des télomères TeloTAGGG, sufficient for ≤50 reactions, kit of 1 (15 components), suitable for cell culture
Sigma-Aldrich
Thioredoxin Reductase Assay Kit, 1 kit sufficient for 100 assays (1 mL)
Sigma-Aldrich
Dihydroethidium, ≥95%