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Using smell to triage samples in point-of-care assays.

Angewandte Chemie (International ed. in English) (2012-10-06)
Hemakesh Mohapatra, Scott T Phillips
RÉSUMÉ

Smell of success: Reagent 1 provides the dual readouts of odor (ethanethiol) and fluorescence (derivative of 7-hydroxycoumarin) and can be used in down-selection assays based on smell and quantitative fluorescence assays of the samples that give a positive result. An important feature of 1 is the matched sensitivity of the two outputs. This reagent is designed for use in resource-limited settings and is demonstrated in assays that detect enzymes.

MATÉRIAUX
Référence du produit
Marque
Description du produit

Sigma-Aldrich
Ethanethiol, 97%
Sigma-Aldrich
Umbelliferone, 99%
Sigma-Aldrich
Sodium ethanethiolate, technical, ~90% (RT)
Sigma-Aldrich
Ethanethiol, ≥97%
Sigma-Aldrich
Sodium ethanethiolate, technical grade, 80%
Sigma-Aldrich
Umbelliferone, suitable for fluorescence indicator, ≥98.0% (HPLC)
Supelco
Umbelliferone, analytical standard