Formation of myo-inositol phosphates by Aspergillus niger 3-phytase.

Kinetics of phytate hydrolysis by Aspergillus niger phytase and correlation between the amount of released phosphate and creation of lower myo-inositol phosphates were investigated. Phytase was able to hydrolyze myo-inositol hexakis-, pentakis-, tetrakis-, and trisphosphates. Finally, about 56% of total phosphate were released and myo-inositol bisphosphate was detected as the end-product.