- Analysis of glycoproteins for biomarker discovery.
Analysis of glycoproteins for biomarker discovery.
Methods in molecular biology (Clifton, N.J.) (2013-04-30)
Jintang He, Yashu Liu, Jing Wu, David M Lubman
PMID23625399
RÉSUMÉ
Glycoproteins play an important role in cell signaling and cell-cell interaction. The alterations of glycoproteins are often relevant to progression of diseases, and these changed glycoproteins can be important biomarkers. The lectin-based glycoproteomic technology has extensively been used for high-throughput screening of potential glycoprotein biomarkers. Here we describe a multi-lectin affinity chromatography and label-free quantitative glycoproteomic approach for discovery of glycoprotein biomarkers relevant to differentiation of glioblastoma stem cells.
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