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Soybean basic 7S globulin: subunit heterogeneity and molecular evolution.

Bioscience, biotechnology, and biochemistry (2010-08-12)
Andrei D Shutov, Krisna Prak, Takako Fukuda, Sergei V Rudakov, Angela S Rudakova, Mary Rose Tandang-Silvas, Keigo Fujiwara, Bunzo Mikami, Shigeru Utsumi, Nobuyuki Maruyama
RÉSUMÉ

Basic 7S globulin, a cysteine-rich protein from soybean seeds, consists of subunits containing 27 kD and 16 kD chains linked by disulfide bonding. Three differently sized subunits of the basic 7S globulin were detected and partially separated by SP Sepharose chromatography. The basic 7S globulin was characterized as a member of a superfamily of structurally related but functionally distinct proteins descended from a specific group of plant aspartic proteinases.

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SP Sepharose, Fast Flow, aqueous ethanol suspension, 45-165 μm (wet), exclusion limit ~4,000,000 Da