SRP4695

ApoA-1 from rat

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonyme(s) : APOA1, Apo-AI, Apolipoprotein A-I, MGC117399
• Numéro CAS: 111366-92-8
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: rat
• Produit recombinant: expressed in E. coli
• Pureté: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Forme: lyophilized
• Poids mol.: ~27.4 kDa
• Conditionnement: pkg of 100 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Impuretés: endotoxin, tested
• Numéro d'accès NCBI: NP_036870
• Numéro d'accès UniProt: P04639
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: rat ... Apoa1(25081)

Description

Description générale

ApoA-I is a 29.0 kDa protein produced in the liver and intestine, and secreted as the predominant constituent of nascent high-density lipoprotein (HDL) particle. ApoA-I, which is found exclusively in HDL, has a unique ability to capture and solubilize free cholesterol. This apoA-I ability enables HDL to remove excess peripheral cholesterol and return it to the liver for recycling and excretion. This process, called reverse cholesterol transport, is though to inhibit atherogenesis. For this reason HDL is also known as the "good cholesterol." The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I (called apoA-I Milano). The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases. Recombinant rat ApoA-I is a 27.4 kDa protein of 235 amino acid residues.

Forme physique

Sterile filtered and Lyophilized without additives.

Notes préparatoires

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitution

Reconstitute in water to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4 °C for 1 week or –20 °C for future use.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3