SAB4200074

Anti-VAC14 antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-ArPIKfyve, Anti-TAX1-binding protein 2, Anti-TAX1BP2, Anti-TAX1BP2 TAX-reactive protein x, Anti-TRX
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~82 kDa
• Espèces réactives: human
• Conditionnement: antibody small pack of 25 μL
• Concentration: ~1.5 mg/mL
• Technique(s): immunoprecipitation (IP): 10-15 μg using A431 cell lysates; indirect immunofluorescence: 8-16 μg/mL using HeLa cells; western blot: 1.5-3.0 μg/mL using HeLa cell lysates
• Numéro d'accès UniProt: Q08AM6
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... VAC14(55697)

Description

Description générale

VAC14 (VAC14 component of PIKFYVE complex) gene (also known as ArPIKfyve, TAX1BP2, TRX) is mapped within human chromosome 16q22.1-q22.2 and encodes a scaffold protein. This protein is a component of phosphoinositide kinase, FYVE-type zinc finger containing (PIKfyve) protein kinase complex.

Application

Anti-VAC14 antibody produced in rabbit has been used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Actions biochimiques/physiologiques

VAC14 is a regulator of phosphoinositide kinase, FYVE-type zinc finger containing (PIP5K3/PIKfyve), a dual specificity kinase that phosphorylates phosphatidylinositol 3-phosphate (PtdIns3P). This generates housekeeping phospholipid PtdIns(3,5)P2, that controls multivesicular body morphology, retrograde traffic to the trans-Golgi network and is critical for neuronal survival. VAC14/ArPIKfyve upregulates PIKfyve phosphoinositide-5-kinase activity. In 3T3-L1 adipocytes, VAC14 and PIP5K3 interaction has been shown to play a critical role in insulin-regulated glucose transporter (GLUT4) translocation from intracellular storage compartment to the cell surface. Knockout of the VAC14 gene in mouse results in massive neurodegeneration and affects neurons in the midbrain and of the peripheral sensory system.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable