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RAB0431

Sigma-Aldrich

Mouse Shh-N ELISA Kit

for serum, plasma and cell culture supernatant

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About This Item

Code UNSPSC :
41116158
Nomenclature NACRES :
NA.32

Espèces réactives

mouse

Conditionnement

kit of 96 wells (12 strips x 8 wells)

Technique(s)

ELISA: suitable
capture ELISA: suitable

Entrée

sample type cell culture supernatant(s)
sample type serum
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 5 pg/mL
standard curve range: 4.1-1000 pg/mL

Méthode de détection

colorimetric

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

mouse ... Shh(20423)

Catégories apparentées

Description générale

The Mouse Shh-N (Sonic Hedgehog N Terminus) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Shh-N in serum, plasma, cell culture supernatants and urine.

Immunogène

Recombinant Mouse Sonic Hedgehog (N-terminus)

Application

For research use only. Not for use in diagnostic procedures.
Mouse Shh-N ELISA Kit has been used to measure serum sonic hedgehog (Shh) level.

Actions biochimiques/physiologiques

Sonic hedgehog (Shh) is a secreted glycoprotein involved in embryonic morphogenic development of several tissues. It has a role in fracture healing and regeneration. In the canonical pathway, the protein associates with its receptor Patched (Ptc-1), de-represses Smoothened (Smo) and activates the Gli family of transcription factors. Mutations in the Shh pathway have been linked to developmental diseases and cancer. Shh plays a vital role in axon growth and guidance throughout the brain development. Shh stimulates neurite outgrowth by overcoming neurite growth inhibitory environment associated with glial scar.

Autres remarques

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

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Description
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  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)FDS
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  • RABWASH420X Wash Buffer (Item B)FDS

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Warning

Mentions de danger

H290

Conseils de prudence

P234 - P390

Classification des risques

Met. Corr. 1

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Sonic hedgehog stimulates neurite outgrowth in a mechanical stretch model of reactive-astrogliosis.
Berretta A
Scientific Reports, 6:21896-6:21896 (2016)
Reduced lipoapoptosis, hedgehog pathway activation and fibrosis in caspase-2 deficient mice with non-alcoholic steatohepatitis.
Machado MV
Gut, 64(7), 1148-1157 (2015)
An epigenetic switch induced by Shh signalling regulates gene activation during development and medulloblastoma growth.
Shi X
Nature Communications, 5:5425-5:5425 (2014)
M V Machado et al.
Gut, 64(7), 1148-1157 (2014-07-24)
Caspase-2 is an initiator caspase involved in multiple apoptotic pathways, particularly in response to specific intracellular stressors (eg, DNA damage, ER stress). We recently reported that caspase-2 was pivotal for the induction of cell death triggered by excessive intracellular accumulation
Lin Ding et al.
Cellular and molecular gastroenterology and hepatology, 11(5), 1251-1266 (2020-12-22)
Helicobacter pylori infection in humans typically begins with colonization of the gastric antrum. The initial Th1 response occasionally coincides with an increase in gastrin secretion. Subsequently, the gastritis segues to chronic atrophic gastritis, metaplasia, dysplasia and distal gastric cancer. Despite
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