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E2886

Sigma-Aldrich

ExtrAvidin®−Peroxidase

buffered aqueous solution

Synonyme(s) :

ExtrAvidin HRP

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About This Item

Numéro MDL:
MFCD00163600
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Conjugué

peroxidase conjugate

Forme

buffered aqueous solution

Technique(s)

direct ELISA: 1:2,000
dot blot: 1:4,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

Conditions d'expédition

wet ice

Température de stockage

2-8°C

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Spécificité

The product is found to be reactive with Anti-Avidin, Catalog Number A5170, and non-reactive with Anti-Streptavidin, Catalog Number S6390, by immunoelectrophoresis.

Application

ExtrAvidin®-Peroxidase is suitable for the following applications:
  • Dot blot at a minimum dilution of 1:4,000
  • Immunohistochemistry (using formalin-fixed, paraffin-embedded sections at a dilution of 1:100)
  • Direct ELISA at a minimum dilution of 1:2,000
It has also been used for:
  • Immunoprecipitation and Immunobloting
  • Ra2I solid-phase binding assays
  • Southwestern blotting
  • Western blotting
  • Immunocytochemistry

Actions biochimiques/physiologiques

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin-Peroxidase may be used in conjunction with biotin in different techniques.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Notes préparatoires

Affinity purified protein

Stockage et stabilité

Store at 2-8 °C. Do Not Freeze. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Informations légales

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H317

Classification des risques

Skin Sens. 1

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

D Tuckwell et al.
Journal of cell science, 108 ( Pt 4), 1629-1637 (1995-04-01)
Integrins alpha 1 beta 1 and alpha 2 beta 1 are major cellular receptors for collagens. The alpha 1 and alpha 2 subunits contain a approximately 200 amino acid inserted domain (I-domain) in their N-terminal region and, because of the
Stefan Lüth et al.
The Journal of clinical investigation, 118(10), 3403-3410 (2008-09-20)
Tregs are important mediators of immune tolerance to self antigens, and it has been suggested that Treg inactivation may cause autoimmune disease. Therefore, immunotherapy approaches that aim to restore or expand autoantigen-specific Treg activity might be beneficial for the treatment
Mohamed S Benleulmi et al.
Retrovirology, 14(1), 54-54 (2017-11-29)
Stable insertion of the retroviral DNA genome into host chromatin requires the functional association between the intasome (integrase·viral DNA complex) and the nucleosome. The data from the literature suggest that direct protein-protein contacts between integrase and histones may be involved
H Kaur et al.
Scandinavian journal of immunology, 62(4), 361-370 (2005-10-29)
The present study was designed to examine the binding and signalling effects of single base and CpG dinucleotide phosphodiester (Po) oligodeoxynucleotides (ODN) on the human natural killer (NK)-like cell line (YT-INDY). Single base Po ODN composed of 20-mers of guanosine
Dimer-tetramer transition between solution and crystalline states of streptavidin and avidin mutants.
Pazy Y, et al.
Journal of Bacteriology, 185(14), 4050-4056 (2003)
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Protocoles

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

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