21888

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

• Synonyme(s) : CFSE
• Formule empirique (notation de Hill): C₂₉H₁₉NO₁₁
• Numéro CAS: 150347-59-4
• Poids moléculaire : 557.46
• Numéro MDL: MFCD00037466
• Code UNSPSC : 12352108
• ID de substance PubChem : 57647951
• Nomenclature NACRES : NA.32

Propriétés

• Gamme de produits: BioReagent
• Pureté: ≥90% (HPLC)
• Forme: solid
• Pf: 152-154 °C (lit.)
• Solubilité: DMF: soluble; DMSO: soluble
• Fluorescence: λ_ex 492 nm; λ_em 517 nm in 0.1 M Tris pH 8.0 (esterase)
• Adéquation: suitable for fluorescence
• Température de stockage: −20°C
• Chaîne SMILES: CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(=O)ON6C(=O)CCC6=O)c1.CC(=O)Oc7ccc8c(Oc9cc(OC(C)=O)ccc9C8%10OC(=O)c%11ccc(cc%10%11)C(=O)ON%12C(=O)CCC%12=O)c7
• InChI: 1S/2C29H19NO11/c1-14(31)37-17-4-7-21-23(12-17)39-24-13-18(38-15(2)32)5-8-22(24)29(21)20-6-3-16(11-19(20)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34;1-14(31)37-17-4-7-20-23(12-17)39-24-13-18(38-15(2)32)5-8-21(24)29(20)22-11-16(3-6-19(22)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h2*3-8,11-13H,9-10H2,1-2H3
• Clé InChI: LZFDYQHOQDFMOA-UHFFFAOYSA-N

Description

Description générale

5-(and-6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is a fluorescent dye, which is membrane permeable. It labels cells by covalently binding to intracellular molecules. CFSE is used to track lymphocyte migration and proliferation.

Application

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is an amine reactive fluorescent probe used to stain cells, especially immune cells, for analysis in applications such as flow cytometry. Forms green fluorescent conjugates on deacetylation.

5-Carboxyfluorescein diacetate N-succinimidyl ester has been used:

• to label cells for flow cytometry
• in lymphocyte proliferation assay
• to determine the intracellular or intra-organellar pH in chloroplasts
• to label Leishmania amazonensis and Leishmania major

Amine-reactive label for long term tracing of cells. Forms green fluorescent conjugates on deacetylation.

Actions biochimiques/physiologiques

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is used as an indicator for esterase activity.

Conditionnement

Bottomless glass bottle. Contents are inside inserted fused cone.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)