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15356

Sigma-Aldrich

Blotting-Nylon 66 membranes, type B, positive

for Northern and Southern blotting

Synonyme(s) :

Nylon 66-Blotting membranes, type B

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About This Item

Code UNSPSC :
41105339
Nomenclature NACRES :
NA.25

Matériaux

Nylon

Qualité

for molecular biology

Forme

roll

Couverture de surface

surface (quaternary ammonium groups) ≥99%

Dimension de pores

0.45 μm pore size

Température de stockage

room temp

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Description générale

Positively charged nylon cast on polyester-support; membrane size 0,2m x 3m (rolled up)

Application

Suitable for:
Protein transfer
Radioactive detection of blotted proteins and nucleic acids
Sensitive dot and slot blots of nucleic acids
for high-sensitive nucleic acid blotting, dot and lot blots. Particularly suitable for radioactive detection. Suitable for protein transfer and immunoblotting.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Gerardas Dambrauskas et al.
Journal of experimental botany, 54(383), 699-706 (2003-01-30)
Very little is known about the expression patterns in plants of genes that encode proteins involved in the initiation of DNA replication. Partial cDNA sequences that encode Cdc6 and Mcm3 in tobacco have been isolated. The sequences were used as
K Pfeifer et al.
Journal of cell science, 106 ( Pt 2), 545-553 (1993-10-01)
Ubiquitination of proteins is a critical step in the controlled degradation process of many polypeptides. Here we show that sponges, the simplest multicellular group of eukaryotic organisms, are also equipped with the ubiquitin pathway. The polyubiquitin cDNA was isolated and

Protocoles

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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