MABC961M

Anti-RTN4IP1 Antibody, clone NIMP‐R14

clone NIMP‐R14, from rat

• Synonyme(s) : Reticulon-4-interacting protein 1, mitochondrial, NOGO-interacting mitochondrial protein
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: rat
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: NIMP‐R14, monoclonal
• Espèces réactives: mouse
• Technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); inhibition assay: suitable
• Isotype: IgG2bκ
• Numéro d'accès NCBI: NP_570962
• Numéro d'accès UniProt: Q924D0
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: mouse ... Rtn4Ip1(170728)

Description

Description générale

Reticulon-4-interacting protein 1, mitochondrial (UniProt: Q924D0; also known as NOGO-interacting mitochondrial protein, RTN4IP1) is encoded by the Rtn4ip1 (also known as Nimp) gene in murine species. RTN4IP1 is an outer mitochondrial membrane protein that interacts with reticulon 4, which is a potent inhibitor of regeneration following spinal cord injury. This interaction may be important for reticulon-induced inhibition of neurite growth. RNT4IP1 plays a role in the regulation of retinal ganglion cell (RGC) neurite outgrowth, and hence in the development of the inner retina and optic nerve. It is widely expressed in mitochondria-enriched tissues and co-localizes with the endoplasmic reticulum HSPA5 at spots corresponding to contacts with mitochondria. Mutations in Rtn4ip1 gene can cause optic atrophy 10 that leads to vision loss sometime in early childhood.

Spécificité

Clone NIMP‐R14 is a rat monoclonal antibody that detects Reticulon-4-interacting protein 1 in murine species.

Immunogène

Purified BALB/c mouse neutrophils.

Application

Anti-RTN4IP1, clone NIMP‐R14, Cat. No. MABC961, is a rat monoclonal antibody that detects Reticulon-4-interacting protein 1, mitochondrial and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Inhibition assays.

Flow Cytometry Analysis: A representative lot detected RTN4IP1 in Flow Cytometry applications (Lopez, A.F., et. al. (1984). Br J Haematol. 57(3):489-94; Taylor, P.R., et. al. (2014). Nat Immunol. 15(2):143-51).

Inhibition Analysis: A representative lot detected RTN4IP1 in Inhibition applications (Tacchini-Cottier, F., et. al. (2000). J Immunol. 165(5):2628-36; de Vries, B., et. al. (2003). J Immunol. 170(7):3883-9; Chang, H.R., et. al. (1993). J Leukoc Biol. 53(6):636-9; Mentzel, S., et. al. (1999). Kidney Int. 55(4):1335-47).

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected RTN4IP1 in mouse lung tissue.

Immunohistochemistry Analysis: A representative lot detected RTN4IP1 in Immunohistochemistry applications (Lopez, A.F., et. al. (1984). Br J Haematol. 57(3):489-94).

Immunohistochemistry Analysis: A representative lot detected RTN4IP1 in Immunohistochemistry applications (Lopez, A.F., et. al. (1984). Br J Haematol. 57(3):489-94.; de Vries, B., et. al. (2003). J Immunol. 170(7):3883-9; Chang, H.R., et. al. (1993). J Leukoc Biol. 53(6):636-9).

Immunocytochemistry Analysis: A representative lot detected RTN4IP1 in Immunocytochemistry applications (Lopez, A.F., et. al. (1984). Br J Haematol. 57(3):489-94.; Taylor, P.R., et. al. (2014). Nat Immunol. 15(2):143-51).

Research Category
Apoptosis & Cancer

Qualité

Evaluated by Immunohistochemistry in mouse spleen tissue.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected RTN4IP1 in mouse spleen tissue.

Description de la cible

43.37 kDa calculated.

Forme physique

Format: Purified

Protein G purified

Purified rat monoclonal antibody IgG2b in PBS without preservatives.

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable