ABT110

Anti-SDHD (CybS) Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial, CybS, CII-4, QPs3, Succinate dehydrogenase complex subunit D, Succinate-ubiquinone oxidoreductase cytochrome b small subunit, Succinate-ubiquinone reductase membrane anchor s
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human, mouse
• Réactivité de l'espèce (prédite par homologie): sheep (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), bovine (based on 100% sequence homology)
• Technique(s): immunocytochemistry: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_002993
• Numéro d'accès UniProt: O14521
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... SDHD(6392)

Description

Description générale

Succinate dehydrogenase complex subunit D (SDHD) is a member of the CybS family and is one of four subunits of the succinate dehydrogenase (SDH) complex involved in the electron transport chain of the mitochondria. The specific role of SDH is to transfer electrons from succinate to ubiquinone, and the SDHD subunit is involved in anchoring the SDH complex to the inner mitochondrial membrane. Defective SDHD has been linked to Carney-Stratakis syndrome, a condition characterized by concurrent paraganglioma and gastric stromal sarcomas.

Spécificité

Other homologies: Rat (92% sequence homology).

This antibody recognizes the Topological domain (mitochondrial matrix) of SDHD (CybS).

Immunogène

Epitope: Topological domain (mitochondrial matrix)

KLH-conjugated linear peptide corresponding to the Topological domain of human SDHD (CybS).

Application

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected SDHD (CybS) in NIH/3T3 and HeLa cells.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

This Anti-SDHD (CybS) Antibody is validated for use in Western Blotting, ICC for the detection of SDHD (CybS).

Qualité

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected SDHD (CybS) in 10 µg of HeLa cell lysate.

Description de la cible

~11 kDa observed. An uncharacterized band may be observed at ~25 kDa in some cell lysates.

Forme physique

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
HeLa cell lysate.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable