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Anti-Phosphotyrosine Antibody, 4G10® Platinum, HRP Conjugate

Upstate®, from mouse, peroxidase conjugate

Synonyme(s) :

phosphotyrosine

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Conjugué

peroxidase conjugate

Type de produit anticorps

primary antibodies

Clone

4G10®, monoclonal
PY20, monoclonal

Réactivité de l'espèce (prédite par homologie)

all

Fabricant/nom de marque

Upstate®

Technique(s)

ELISA: suitable
western blot: suitable

Isotype

IgG2b

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

phosphorylation (pTyr)

Informations sur le gène

human ... PID1(55022)

Description générale

The development of the anti-phosphotyrosine, clone 4G10 in 1989 was a monumental discovery for researchers. 4G10 was the first and is the best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10 is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. To improve on something that hundred have tried and no one has succeeded, we pooled 4G10 with the next most highly regarded anti-phosphotyrosine, clone PY20 to make 4G10 Platinum. PY20 itself is a very poor substitute for 4G10, but its additive effect allow for a greater level of detection on more substrates that even 4G10 alone.

Application

Detect Phosphotyrosine with Anti-Phosphotyrosine Antibody, 4G10 Platinum, HRP Conjugate (Mouse Monoclonal Antibody), that has been shown to work in WB, ELISA.

Qualité

Routinely evaluated by western blot.

Description de la cible

Varies

Forme physique

100 μL of a proprietary mixture of protein G purified mouse monoclonal 4G10 (IgG2bƙ) HRP conjugate and protein A purified mouse monoclonal PY20, HRP conjugate (IgG2b) in PBS containing 0.05% Kathon and HRP stabilizing compounds. Sufficient for a minumum of 10 immunoblots, if used at the optimum concentration of 1:2000 – 1:4000 in 10 mL blocking buffer. Liquid.

Informations légales

4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Classification des risques

Aquatic Chronic 2 - Skin Sens. 1

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Irmgard Hofmann et al.
The Journal of pharmacology and experimental therapeutics (2022-10-15)
VEGF and angiopoietin-2 (ANG2) have complementary roles in angiogenesis and promote an immunosuppressive tumor microenvironment. It is anticipated that combination of VEGF and ANG2 blockade could provide superior activity to blockade of either pathway alone, and that addition of VEGF/ANG2
T Wang et al.
Nihon Rai Gakkai zasshi, 60(3-4), 146-151 (1991-07-01)
A comparative study on the usefulness of Kawatsu's silver impregnating staining method compared with S-100 protein ABC technique for the differential diagnosis of tuberculoid leprosy was carried out. The results of neurohistological examination obtained from both methods were almost the
Ondřej Ballek et al.
Immunology letters, 142(1-2), 64-74 (2012-01-28)
Lck is the principal signal-generating tyrosine kinase of the T cell activation mechanism. We have previously demonstrated that induced Lck activation outside of lipid rafts (LR) results in the rapid translocation of a fraction of Lck to LR. While this
Wei Tian et al.
BMC biochemistry, 13, 22-22 (2012-10-25)
Phosphatase of regenerating liver-3 (PRL-3 or PTP4A3) has been implicated in controlling cancer cell proliferation, motility, metastasis, and angiogenesis. Deregulated expression of PRL-3 is highly correlated with cancer progression and predicts poor survival. Although PRL-3 was categorized as a tyrosine
Guillermina M Goñi et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(31), E3177-E3186 (2014-07-23)
Focal adhesion kinase (FAK) is a nonreceptor tyrosine kinase (NRTK) with key roles in integrating growth and cell matrix adhesion signals, and FAK is a major driver of invasion and metastasis in cancer. Cell adhesion via integrin receptors is well

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