1.25137

Fractogel^® EMD TMAE Hicap (M)

RoboColumn Fractogel^® TMAE Hicap (M), volume 0.6 mL, prepacked column

• Synonyme(s) : RoboColumn Fractogel^® TMAE Hicap (M), 0.6 ml
• Code UNSPSC : 41115711

Propriétés

• ligand: (Trimethylammoniumethyl)
• Matériaux: polypropylene housing
• Gamme de produits: Fractogel^®
• Forme: prepacked column
• Paramètres: ≤8 bar pressure; ~180 cm/hr max. flow rate
• column I.D. × L: 5 mm × 30 mm
• Volume: 0.6 mL
• Groupe de la matrice active: Methacrylate
• Taille moyenne des particules: 40-90 μm
• Capacité: ~180 mg, BSA binding capacity
• Application(s): mAb; recombinant protein; vaccine development
• Technique de séparation: anion exchange
• Température de stockage: 15-25°C

Description

Description générale

5 x 30 mm

Caractéristiques et avantages

RoboColumn Fractogel^® TMAE Hicap (M), 0.6 ml enables users to:

• Efficiently capture target protein and remove viruses, DNA, and endotoxins with its tentacle technology resulting in better production yields and high throughput
• Reduce operating costs and resin replacement with enduring durability - allowing for multiple cycles of column regeneration and sanitization
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior of Fractogel^® EMD TMAE Hicap (M) the ion exchange capacity can be used from pH 2 to 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel^® EMD TMAE Hicap (M) when the pH of the buffer is about 1 unit above their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

Informations légales

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany