Minimum Essential Medium Eagle (MEM) Formulation

Minimum Essential Medium Eagle (MEM) Alpha Modification
Minimum Essential Medium Eagle (MEM) HEPES Modification
Minimum Essential Medium Eagle (MEM) Joklik Modification for Suspension Cultures
Minimum Essential Medium Eagle (MEM) Modified
Minimum Essential Medium Eagle (MEM) Auto-Mod™ Modified for Autoclaving
Minimum Essential Medium Eagle (S-MEM) Spinner Modification

Minimum Essential Medium Eagle (MEM)

Component	M0268 g/L	M0275 [10x] g/L	M0446 [1x] g/L	M0643 g/L	M1018 g/L
Inorganic Salts
Calcium Chloride	0.2	2	0.2	0.2	0.1396
Magnesium Sulfate (anhydrous)	0.09767	0.9767	0.09767	0.09767	0.09767
Potassium Chloride	0.4	4	0.4	0.4	0.4
Potassium Phosphate Monobasic (anhydrous)	—	—	—	—	0.06
Sodium Bicarbonate	—	—	2.2	—	—
Sodium Chloride	6.8	68	6.8	6.8	8
Sodium Phosphate Dibasic (anhydrous)	—	—	—	—	0.04788
Sodium Phosphate Monobasic (anhydrous)	0.122	1.22	0.122	0.122	—
Amino Acids
L-Alanine	—	—	—	0.0089	0.0089
L-Alanyl-L-Glutamine	—	—	0.4344	—	—
L-Arginine • HCl	0.126	1.26	0.126	0.126	0.126
L-Asparagine • H2O	—	—	—	0.015	0.015
L-Aspartic Acid	—	—	—	0.0133	0.0133
L-Cystine • 2HCl	0.0313	0.313	0.0313	0.0313	0.0313
L-Glutamic Acid	—	—	—	0.0147	0.0147
L-Glutamine	0.292	—	0.292	0.292	0.292
Glycine	—	—	—	0.0075	0.0075
L-Histidine • HCl • H2O	0.042	0.42	0.042	0.042	0.042
L-Isoleucine	0.052	0.52	0.052	0.052	0.052
L-Leucine	0.052	0.52	0.052	0.052	0.052
L-Lysine • HCl	0.0725	0.725	0.0725	0.0725	0.0725
L-Methionine	0.015	0.15	0.015	0.015	0.015
L-Phenylalanine	0.032	0.32	0.032	0.032	0.032
L-Proline	—	—	—	0.0115	0.0115
L-Serine	—	—	—	0.0105	0.0105
L-Threonine	0.048	0.48	0.048	0.048	0.048
L-Tryptophan	0.01	0.1	0.01	0.01	0.01
L-Tyrosine • 2Na • 2H2O	0.0519	0.519	0.0519	0.0519	0.0519
L-Valine	0.046	0.46	0.046	0.046	0.046
Vitamins
Choline Chloride	0.001	0.01	0.001	0.001	0.001
Folic Acid	0.001	0.01	0.001	0.001	0.001
myo-Inositol	0.002	0.02	0.002	0.002	0.002
Niacinamide	0.001	0.01	0.001	0.001	0.001
D-Pantothenic Acid (hemicalcium)	0.001	0.01	0.001	0.001	0.001
Pyridoxal • HCl	0.001	0.01	0.001	0.001	0.001
Riboflavin	0.0001	0.001	0.0001	0.0001	0.0001
Thiamine • HCl	0.001	0.01	0.001	0.001	0.001
Other
Glucose	1	10	1	1	1
Phenol Red • Na	0.011	0.11	0.011	0.011	0.011
Add
L-Glutamine	—	0.292 at 1×	—	—	—
Sodium Bicarbonate	2.2	2.2 at 1×	—	2.2	0.35

Minimum Essential Medium Eagle (MEM) (cont.)

Component	M2279 g/L	M2414 [1x] g/L	M4642 [1x] g/L	M4655 g/L
Inorganic Salts
Calcium Chloride	0.2	0.2	0.2	0.2
Magnesium Sulfate (anhydrous)	0.09767	0.09767	0.09767	0.09767
Potassium Chloride	0.4	0.4	0.4	0.4
Potassium Phosphate Monobasic (anhydrous)	—	—	0.06	—
Sodium Bicarbonate	2.2	2.2	—	2.2
Sodium Chloride	6.8	6.8	8	6.8
Sodium Phosphate Dibasic (anhydrous)	—	—	0.04788	—
Sodium Phosphate Monobasic (anhydrous)	0.122	0.122	—	0.122
Amino Acids
L-Alanine	—	—	—	—
L-Arginine • HCl	0.126	0.126	0.126	0.126
L-Asparagine • H2O	—	—	—	—
L-Aspartic Acid	—	—	—	—
L-Cystine • 2HCl	0.0313	0.0313	0.0313	0.0313
L-Glutamic Acid	—	—	—	—
L-Glutamine	—	0.292	0.292	0.292
Glycine	—	—	—	—
L-Histidine • HCl • H2O	0.042	0.042	0.042	0.042
L-Isoleucine	0.052	0.052	0.052	0.052
L-Leucine	0.052	0.052	0.052	0.052
L-Lysine • HCl	0.0725	0.0725	0.0725	0.0725
L-Methionine	0.015	0.015	0.015	0.015
L-Phenylalanine	0.032	0.032	0.032	0.032
L-Proline	—	—	—	—
L-Serine	—	—	—	—
L-Threonine	0.048	0.048	0.048	0.048
L-Tryptophan	0.01	0.01	0.01	0.01
L-Tyrosine • 2Na • 2H2O	0.0519	0.0519	0.0519	0.0519
L-Valine	0.046	0.046	0.046	0.046
Vitamins
Choline Chloride	0.001	0.001	0.001	0.001
Folic Acid	0.001	0.001	0.001	0.001
myo-Inositol	0.002	0.002	0.002	0.002
Niacinamide	0.001	0.001	0.001	0.001
D-Pantothenic Acid (hemicalcium)	0.001	0.001	0.001	0.001
Pyridoxal • HCl	0.001	0.001	0.001	0.001
Riboflavin	0.0001	0.0001	0.0001	0.0001
Thiamine • HCl	0.001	0.001	0.001	0.001
Other
Glucose	1	1	1	1
Phenol Red • Na	0.011	0.011	0.011	0.011
Add
L-Glutamine	0.292	—	—	—
Sodium Bicarbonate	—	—	0.35	—

Minimum Essential Medium Eagle (MEM) (cont.)

Component	M4780 [1x] g/L	M5650 [1x] g/L	M5775 [1x] g/L	M9288 [10x] g/L
Inorganic Salts
Calcium Chloride	0.1396	0.2	0.1396	1.396
Magnesium Sulfate (anhydrous)	0.09767	0.09767	0.09767	0.9767
Potassium Chloride	0.4	0.4	0.4	4
Potassium Phosphate Monobasic (anhydrous)	0.06	—	0.06	0.6
Sodium Bicarbonate	0.35	2.2	0.35	—
Sodium Chloride	8	6.8	8	80
Sodium Phosphate Dibasic (anhydrous)	0.04788	—	0.04788	0.4788
Sodium Phosphate Monobasic (anhydrous)	—	0.122	—	—
Amino Acids
L-Alanine	—	0.0089	—	—
L-Arginine • HCl	0.126	0.126	0.126	1.26
L-Asparagine • H2O	—	0.015	—	—
L-Aspartic Acid	—	0.0133	—	—
L-Cystine • 2HCl	0.0313	0.0313	0.0313	0.313
L-Glutamic Acid	—	0.0147	—	—
L-Glutamine	0.292	—	—	—
Glycine	—	0.0075	—	—
L-Histidine • HCl • H2O	0.042	0.042	0.042	0.42
L-Isoleucine	0.052	0.052	0.052	0.52
L-Leucine	0.052	0.052	0.052	0.52
L-Lysine • HCl	0.0725	0.0725	0.0725	0.725
L-Methionine	0.015	0.015	0.015	0.15
L-Phenylalanine	0.032	0.032	0.032	0.32
L-Proline	—	0.0115	—	—
L-Serine	—	0.0105	—	—
L-Threonine	0.048	0.048	0.048	0.48
L-Tryptophan	0.01	0.01	0.01	0.1
L-Tyrosine • 2Na • 2H2O	0.0519	0.0519	0.0519	0.519
L-Valine	0.046	0.046	0.046	0.46
Vitamins
Choline Chloride	0.001	0.001	0.001	0.01
Folic Acid	0.001	0.001	0.001	0.01
myo-Inositol	0.002	0.002	0.002	0.02
Niacinamide	0.001	0.001	0.001	0.01
D-Pantothenic Acid (hemicalcium)	0.001	0.001	0.001	0.01
Pyridoxal • HCl	0.001	0.001	0.001	0.01
Riboflavin	0.0001	0.0001	0.0001	0.001
Thiamine • HCl	0.001	0.001	0.001	0.01
Other
Glucose	1	1	1	10
Phenol Red • Na	0.011	0.011	0.011	0.11
Add
L-Glutamine	—	0.292	0.292	0.292 at 1×
Sodium Bicarbonate	—	—	—	0.35 at 1×

Minimum Essential Medium Eagle (MEM) Alpha Modification

Component	M0644 g/L	M0894 g/L	M4526 [1x] g/L	M8042 [1x] g/L
Inorganic Salts
Calcium Chloride	0.2	0.2	0.2	0.2
Magnesium Sulfate (anhydrous)	0.09767	0.09767	0.09767	0.09767
Potassium Chloride	0.4	0.4	0.4	0.4
Sodium Bicarbonate	—	—	2.2	2.2
Sodium Chloride	6.8	6.8	6.8	6.8
Sodium Phosphate Monobasic (anhydrous)	0.122	0.122	0.122	0.122
Amino Acids
L-Alanine	0.025	0.025	0.025	0.025
L-Arginine • HCl	0.126	0.126	0.126	0.126
L-Asparagine • H2O	0.05	0.05	0.05	0.05
L-Aspartic Acid	0.03	0.03	0.03	0.03
L-Cysteine • HCl • H2O	0.1	0.1	0.1	0.1
L-Cystine • 2HCl	0.0313	0.0313	0.0313	0.0313
L-Glutamic Acid	0.075	0.075	0.075	0.075
L-Glutamine	0.292	0.292	—	—
Glycine	0.05	0.05	0.05	0.05
L-Histidine • HCl • H2O	0.042	0.042	0.042	0.042
L-Isoleucine	0.052	0.052	0.052	0.052
L-Leucine	0.052	0.052	0.052	0.052
L-Lysine • HCl	0.0725	0.0725	0.0725	0.0725
L-Methionine	0.015	0.015	0.015	0.015
L-Phenylalanine	0.032	0.032	0.032	0.032
L-Proline	0.04	0.04	0.04	0.04
L-Serine	0.025	0.025	0.025	0.025
L-Threonine	0.048	0.048	0.048	0.048
L-Tryptophan	0.01	0.01	0.01	0.01
L-Tyrosine • 2Na • 2H2O	0.0519	0.0519	0.0519	0.0519
L-Valine	0.046	0.046	0.046	0.046
Vitamins
L-Ascorbic Acid • Na	0.05	0.05	0.05	0.05
Biotin	0.0001	0.0001	0.0001	0.0001
Choline Chloride	0.001	0.001	0.001	0.001
Folic Acid	0.001	0.001	0.001	0.001
myo-Inositol	0.002	0.002	0.002	0.002
Lipoic Acid	—	—	0.0002	—
Niacinamide	0.001	0.001	0.001	0.001
D-Pantothenic Acid (hemicalcium)	0.001	0.001	0.001	0.001
Pyridoxal • HCl	0.001	0.001	0.001	0.001
Riboflavin	0.0001	0.0001	0.0001	0.0001
Thiamine • HCl	0.001	0.001	0.001	0.001
Vitamin B12	0.00136	0.00136	0.00136	0.00136
Other
Adenosine	0.01	—	—	0.01
Cytidine	0.01	—	—	0.01
2'-Deoxyadenosine	0.01	—	—	0.01
2'-Deoxycytidine • HCl	0.011	—	—	0.011
2'-Deoxyguanosine	0.01	—	—	0.01
Glucose	1	1	1	1
Guanosine	0.01	—	—	0.01
Phenol Red • Na	0.011	0.011	0.011	0.011
Pyruvic Acid • Na	0.11	0.11	0.11	0.11
Thioctic Acid	0.0002	0.0002	—	0.0002
Thymidine	0.01	—	—	0.01
Uridine	0.01	—	—	0.01
Add
L-Glutamine	—	—	0.292	0.292
Sodium Bicarbonate	2.2	2.2	—	—

Minimum Essential Medium Eagle (MEM) HEPES Modification

Component	M2645 g/L	M7278 [1x] g/L
Inorganic Salts
Calcium Chloride	0.2	0.2
Magnesium Sulfate (anhydrous)	0.09767	0.09767
Potassium Chloride	0.4	0.4
Sodium Bicarbonate	—	2.2
Sodium Chloride	5.5	5.5
Sodium Phosphate Monobasic (anhydrous)	0.122	0.122
Amino Acids
L-Arginine • HCl	0.126	0.126
L-Cystine • 2HCl	0.0313	0.0313
L-Glutamine	0.292	—
L-Histidine • HCl • H2O	0.042	0.042
L-Isoleucine	0.052	0.052
L-Leucine	0.052	0.052
L-Lysine • HCl	0.0725	0.0725
L-Methionine	0.015	0.015
L-Phenylalanine	0.032	0.032
L-Threonine	0.048	0.048
L-Tryptophan	0.01	0.01
L-Tyrosine • 2Na • 2H2O	0.0519	0.0519
L-Valine	0.046	0.046
Vitamins
Choline Chloride	0.001	0.001
Folic Acid	0.001	0.001
myo-Inositol	0.002	0.002
Niacinamide	0.001	0.001
D-Pantothenic Acid (hemicalcium)	0.001	0.001
Pyridoxal • HCl	0.001	0.001
Riboflavin	0.0001	0.0001
Thiamine • HCl	0.001	0.001
Other
Glucose	1	1
HEPES	5.958	5.958
Phenol Red • Na	0.011	0.011
Add
L-Glutamine	—	0.292
Sodium Bicarbonate	2.2	—

Minimum Essential Medium Eagle (MEM) Joklik Modification for Suspension Cultures

Component	M0518 g/L	M8028 [1x] g/L
Inorganic Salts
Magnesium Chloride • 6H2O	0.2	0.2
Potassium Chloride	0.4	0.4
Sodium Bicarbonate	—	2
Sodium Chloride	6.5	6.5
Sodium Phosphate Monobasic (anhydrous)	1.154	1.154
Amino Acids
L-Arginine • HCl	0.126	1.26
L-Cystine • 2HCl	0.0324	0.0324
L-Glutamine	0.292	—
L-Histidine • HCl • H2O	0.042	0.042
L-Isoleucine	0.052	0.052
L-Leucine	0.052	0.052
L-Lysine • HCl	0.0725	0.0725
L-Methionine	0.015	0.015
L-Phenylalanine	0.032	0.032
L-Threonine	0.048	0.048
L-Tryptophan	0.01	0.01
L-Tyrosine • 2Na • 2H2O	0.05452	0.05452
L-Valine	0.046	0.046
Vitamins
Choline Chloride	0.001	0.001
Folic Acid	0.001	0.001
myo-Inositol	0.002	0.002
Niacinamide	0.001	0.001
D-Pantothenic Acid (hemicalcium)	0.001	0.001
Pyridoxal • HCl	0.001	0.001
Riboflavin	0.0001	0.0001
Thiamine • HCl	0.001	0.001
Other
Glucose	2	2
Phenol Red • Na	0.011	0.011
Add
L-Glutamine	—	0.292
Sodium Bicarbonate	2	—

Minimum Essential Medium Eagle (MEM) Modified

Component	M3024 g/L
Inorganic Salts
Calcium Chloride	0.2
Magnesium Sulfate (anhydrous)	0.09767
Potassium Chloride	0.4
Sodium Chloride	6.8
Sodium Phosphate Monobasic (anhydrous)	0.122
Amino Acids
L-Alanine	0.0089
L-Arginine • HCl	0.126
L-Asparagine • H2O	0.015
L-Aspartic Acid	0.0133
L-Cystine • 2HCl	0.0313
L-Glutamic Acid	0.0147
Glycine	0.0075
L-Histidine • HCl • H2O	0.042
L-Isoleucine	0.052
L-Leucine	0.052
L-Lysine • HCl	0.0725
L-Methionine	0.015
L-Phenylalanine	0.032
L-Proline	0.0115
L-Serine	0.0105
L-Threonine	0.048
L-Tryptophan	0.01
L-Tyrosine • 2Na • 2H2O	0.0519
L-Valine	0.046
Vitamins
Choline Chloride	0.001
Folic Acid	0.001
myo-Inositol	0.002
Niacinamide	0.001
D-Pantothenic Acid (hemicalcium)	0.001
Pyridoxal • HCl	0.001
Riboflavin	0.0001
Thiamine • HCl	0.001
Other
Glucose	1
Add
L-Glutamine	0.292
Sodium Bicarbonate	2.2

Minimum Essential Medium Eagle (MEM) Auto-Mod™ Modified for Autoclaving

Component	M0769 g/L
Inorganic Salts
Calcium Chloride	0.2
Magnesium Sulfate (anhydrous)	0.09767
Potassium Chloride	0.4
Sodium Chloride	6.8
Sodium Phosphate Monobasic (anhydrous)	0.122
Sodium Succinate • 6H2O	0.1
Succinic Acid (free acid)	0.075
Amino Acids
L-Arginine • HCl	0.126
L-Cystine • 2HCl	0.0313
L-Histidine • HCl • H2O	0.042
L-Isoleucine	0.052
L-Leucine	0.052
L-Lysine • HCl	0.0725
L-Methionine	0.015
L-Phenylalanine	0.032
L-Threonine	0.048
L-Tryptophan	0.01
L-Tyrosine (free base)	0.036
L-Valine	0.046
Vitamins
Choline Bitartrate	0.0018
Folic Acid	0.001
myo-Inositol	0.002
Niacinamide	0.001
D-Pantothenic Acid (hemicalcium)	0.001
Pyridoxal • HCl	0.001
Riboflavin	0.0001
Thiamine • HCl	0.001
Other
Glucose	1
Phenol Red • Na	0.0064
Add
L-Glutamine	0.292
Sodium Bicarbonate	2.2

Minimum Essential Medium Eagle (S-MEM) Spinner Modification

Component	M8167 [1x] g/L
Inorganic Salts
Magnesium Sulfate (anhydrous)	0.09767
Potassium Chloride	0.4
Sodium Chloride	6.8
Sodium Phosphate Monobasic (anhydrous)	0.122
Amino Acids
L-Arginine • HCl	0.126
L-Cystine • 2HCl	0.0313
L-Histidine • HCl • H2O	0.042
L-Isoleucine	0.052
L-Leucine	0.052
L-Lysine • HCl	0.0725
L-Methionine	0.015
L-Phenylalanine	0.032
L-Threonine	0.048
L-Tryptophan	0.01
L-Tyrosine • 2Na • 2H2O	0.0519
L-Valine	0.046
Vitamins
Choline Chloride	0.001
Folic Acid	0.001
myo-Inositol	0.002
Niacinamide	0.001
D-Pantothenic Acid (hemicalcium)	0.001
Pyridoxal • HCl	0.001
Riboflavin	0.0001
Thiamine • HCl	0.001
Other
Glucose	1
Phenol Red • Na	0.011
Sodium Bicarbonate	2.2
Add
L-Glutamine	0.292