UHPLC Gradient Analysis of Boswellia Herbal Supplement on Ascentis® Express Phenyl-Hexyl
CONDITIONS
column
Ascentis Express Phenyl-Hexyl, 10 cm x 3.0 mm I.D., 2.7 μm particles (53345-U)
mobile phase
[A] 0.1% phosphoric acid; [B] acetonitrile
gradient
50% B for 0.5 min; to 100% B in 4.5 min; held at 100% B for 5 min
gradient
0.6 mL/min
pressure
2390 psi (165 bar)
column temp.
35 °C
detector
UV, 210 nm
injection
2 μL
Description
Application
extraction:
1. Weigh 100 mg Bosewellia Herbal Supplement
2. Add 10 mL ethanol
3. Sonicate 30 min at 40°C
4. Dilute 100 μL extract, 400 μL ethanol, 500 μL water
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany