TLC Analysis Of Plant Extracts on HPTLC Silica gel 60 NH2 F254s
Materials
accessory
Product No.
Description
Pricing
Hamilton® TLC syringes
1702, volume 25 μL, needle size 22s ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1705, volume 50 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1710, volume 100 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
CONDITIONS
sample preparation
2 g plant material extracted for 1 h at 60°C under shaking with 50 ml solvent. Filtration of crude extract with an 0.45 μm syringe filter. Application by CAMAG ATS4 6 mm bandwise.
plate
1.15647 HPTLC Silica gel 60 RP-18 F 254s MS-grade 20cm x 10 cm (1.151647.0001)
eluent
[A] ethylacetat; [B] formic acid; [C] glacial acid; [D] water; (100:11:11:27, A:B:C:D) containing 0.1% TFA
migration distance
6 cm
chamber
normal chamber without chamber saturation
sample
0.150 μL
detector
UV, 254 nm (Identification by coupling the TLC – MS Interface (CAMAG) with the Advion expression) (CMS mass spectrometer (+)-ESI mode. Eluent: Acetonitrile / Water 95:5 + 0.1% TFA.)