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LC/MS Analysis of Vitamin D2, D3, Gamma- and Alpha-Tocopherol on Ascentis® Express C18

LC/MS Analysis of Vitamin D2, D3, Gamma- and Alpha-Tocopherol on Ascentis® Express C18 application for HPLC

Materials

analytical column

Product No.
Description
Pricing

53823-U

Ascentis® Express C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

standard

Product No.
Description
Pricing

T3634

(+)-α-Tocopherol

from vegetable oil, Type V, ~1000 IU/g

47785

(+)-γ-Tocopherol

analytical standard

740292

Vitamin D3 solution

100 μg/mL in ethanol, 97% (CP)

740306

Vitamin D2 solution

100 μg/mL in ethanol, 98% (CP)

CONDITIONS

column

Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)

mobile phase

[A] water; [B] acetonitrile; (5:95, A:B)

flow rate

0.4 mL/min

column temp.

40 °C

detector

MS, ESI+

injection

2 μL

sample

1 μg/mL in acetonitrile

Description

Analysis Note

Analysis was conducted using standard solution, detection on LC/MS system.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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