Higher Efficiency of Ascentis^® Express 2.7 μm Compared to 3 μm Particles Gives Better Sensitivity

column

Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 μm particles and C18, 5 cm x 2.1 mm I.D., 3 μm particles (53822-U)

mobile phase

[A] 25 mM dibasic ammonium phosphate (pH 7.0): [B] water: [C] acetonitrile; (35:0:65 or 35:4:61, A:B:C)

flow rate

0.2 mL/min

column temp.

35 °C

detector

UV, 220 nm

injection

1 μL