HPLC Analysis of Superoxide Dismutase on Discovery® BIO PolyMA-WAX
Materials
standard
Product No.
Description
Pricing
Superoxide Dismutase from bovine erythrocytes
BioReagent, ≥3,000 units/mg protein, lyophilized powder, suitable for cell cultureSuperoxide Dismutase from bovine liver
ammonium sulfate suspension, 2,000-6,000 units/mg protein (biuret)Superoxide Dismutase from bovine erythrocytes
lyophilized powder, ≥3,000 units/mg protein, Protein ≥95 % by biuretSuperoxide Dismutase from bovine erythrocytes
BioUltra, lyophilized powder, ≥4,500 units/mg protein, ≥97% (SDS-PAGE)CONDITIONS
column
Discovery BIO PolyMA-WAX, 5 cm × 4.6 mm, 5 μm particles (59602-U)
mobile phase
([A] 20 mM Tris, pH 8.0 with hydrocloric acid; [B] 20 mM Tris, 0.5 M sodium chloride, pH 8.0 with hydrochloric acid)
gradient
0 to 10% B in 30 min. (linear)
flow rate
0.5 mL/min
column temp.
25 °C
detector
UV, 280 nm
injection
10 μL
Description
Analysis Note
Discovery BIO PolyMA-SCX and PolyMA-WAX columns give sharp, efficient peaks for a wide variety of proteins.
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany