HPLC Analysis of Hydrophobic Bases on Ascentis^® RP-Amide

column

Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565324-U)

mobile phase

[A] 25 mM ammonium phosphate, pH 7; [B] methanol; (35:65, A:B)

flow rate

1.2 mL/min

column temp.

35 °C

detector

UV, 230 nm

injection

10 μL

sample

N05158