HPLC Analysis of Human Growth Hormone (hGH) on Discovery® BIO PolyMA-WAX
Materials
standard
Product No.
Description
Pricing
Human Growth Hormone human
HGH, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell cultureCONDITIONS
column
Discovery BIO PolyMA-WAX, 5 cm × 4.6 mm I.D., 5 μm particles (59602-U)
mobile phase
[A] 20 mM Tris-HCl, pH 8.0; [B] 20 mM Tris-HCl, 0.5 M NaCl, pH 8.0
gradient
5 to 70% B in 30 min. (linear)
flow rate
0.5 mL/min
column temp.
25 °C
detector
UV, 210 nm
injection
10 mg hGH
Description
Analysis Note
This Figure shows the degradation products of human growth hormone (hGH) well-separated on a Discovery BIO PolyMA-WAX anion-exchange column. The difference between the resolved compounds is the conversion of protein amide(s) to carboxylate(s), demonstrating both the power of the ion-exchange technique, and the efficiency of the Discovery BIO PolyMA-WAX column.
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany