HPLC Analysis of Column Test Mix on Discovery^® BIO Wide Pore C18, Effect of HPLC Column I.D. on Sensitivity

column

Discovery BIO Wide Pore C18, 10 cm x various I.D., 3 μm particles

mobile phase

[A] water; [B] acetonitrile; (65:35, A:B)

flow rate

as indicated

column temp.

ambient

detector

UV, 254 nm

injection

as indicated

sample

acetophenone (0.1 μg), benzene (1 μg), toluene (1 μg)