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HPLC Analysis of Carotenes on Ascentis® Express RP-Amide, Methanol Mobile Phase

HPLC Analysis of Carotenes on Ascentis® Express RP-Amide, Methanol Mobile Phase application for HPLC

Materials

analytical column

Product No.
Description
Pricing

53931-U

Ascentis® Express RP-Amide, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

standard

Product No.
Description
Pricing

C4582

β-Carotene

synthetic, ≥95% (HPLC), crystalline

CONDITIONS

column

Ascentis Express RP-Amide, 15 cm x 4.6 mm I.D., 2.7 μm particles (53931-U)

mobile phase

methanol

flow rate

1 mL/min

pressure

1653 psi (114 bar)

column temp.

30 °C

detector

UV-VIS, 454 nm

injection

5 μL

sample

V8 Splash® fruit juice was extracted with hexane, the extract dissolved 1:1 into ethanol for HPLC injection

Description

Analysis Note

Analysis was conducted using juice extract, detection via UV.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
V8 Splash is a registered trademark of CSC Brands, LP

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