HPLC Analysis MAb Variants on Proteomix® SCX-NP5, Fast Analysis
Materials
analytical column
Product No.
Description
Pricing
Proteomix® SCX-NP5 Strong Cation Exchange Column
L × I.D. 25 cm × 4.6 mm, 5 μm particle size, PEEKCONDITIONS
column
Proteomix SCX-NP5, 25 cm x 4.6 mm I.D., 5 μm particles (Z777164)
mobile phase
[A] 2.4 mM Tris, 1.5 mM imidazole, 11.6 mM piperazine, pH 6.0; [B] A + 0.5 M NaCl, pH 10.5
gradient
top (0.8 mL/min): 5% B held for 5 min; to 29.5% B in 35 min; bottom (1.5 mL/min): 5% B held for 2.7 min; to 29.5% B in 18.6 min
flow rate
0.8 mL/min or 1.5 mL/min
detector
UV 280 nm
injection
20 μL
sample
MAb 321, 5 mg/mL
Description
Analysis Note
Due to the low pressure of Proteomix SCX, faster analysis and high resolution can be achieved with a higher flow rate.
Legal Information
Proteomix is a registered trademark of Sepax Technologies