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  • The L1TD1 protein interactome reveals the importance of post-transcriptional regulation in human pluripotency.

The L1TD1 protein interactome reveals the importance of post-transcriptional regulation in human pluripotency.

Stem cell reports (2015-02-24)
Maheswara Reddy Emani, Elisa Närvä, Aki Stubb, Deepankar Chakroborty, Miro Viitala, Anne Rokka, Nelly Rahkonen, Robert Moulder, Konstantin Denessiouk, Ras Trokovic, Riikka Lund, Laura L Elo, Riitta Lahesmaa
ABSTRACT

The RNA-binding protein L1TD1 is one of the most specific and abundant proteins in pluripotent stem cells and is essential for the maintenance of pluripotency in human cells. Here, we identify the protein interaction network of L1TD1 in human embryonic stem cells (hESCs) and provide insights into the interactome network constructed in human pluripotent cells. Our data reveal that L1TD1 has an important role in RNA splicing, translation, protein traffic, and degradation. L1TD1 interacts with multiple stem-cell-specific proteins, many of which are still uncharacterized in the context of development. Further, we show that L1TD1 is a part of the pluripotency interactome network of OCT4, SOX2, and NANOG, bridging nuclear and cytoplasmic regulation and highlighting the importance of RNA biology in pluripotency.

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Normal Rabbit IgG, Normal Rabbit IgG Polyclonal Antibody control validated for use in Immunoprecipitation & Western Blotting.