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  • Development of an ELISA assay for the quantification of soluble huntingtin in human blood cells.

Development of an ELISA assay for the quantification of soluble huntingtin in human blood cells.

BMC biochemistry (2013-11-28)
Luisa Massai, Lara Petricca, Letizia Magnoni, Luca Rovetini, Salman Haider, Ralph Andre, Sarah J Tabrizi, Sigurd D Süssmuth, Bernhard G Landwehrmeyer, Andrea Caricasole, Giuseppe Pollio, Simonetta Bernocco
ABSTRACT

Huntington's disease (HD) is a monogenic disorder caused by an aberrant expansion of CAG repeats in the huntingtin gene (HTT). Pathogenesis is associated with expression of the mutant (mHTT) protein in the CNS, with its levels most likely related to disease progression and symptom severity. Since non-invasive methods to quantify HTT in the CNS do not exist, measuring amount of soluble HTT in peripheral cells represents an important step in development of disease-modifying interventions in HD. An ELISA assay using commercially available antibodies was developed to quantify HTT levels in complex matrices like mammalian cell cultures lysates and human samples. The immunoassay was optimized using a recombinant full-length HTT protein, and validated both on wild-type and mutant HTT species. The ability of the assay to detect significant variations of soluble HTT levels was evaluated using an HSP90 inhibitor that is known to enhance HTT degradation. Once optimized, the bioassay was applied to peripheral blood mononuclear cells (PBMCs) from HD patients, demonstrating good potential in tracking the disease course. The method described here represents a validated, simple and rapid bio-molecular assay to evaluate soluble HTT levels in blood cells as useful tool in disease and pharmacodynamic marker identification for observational and clinical trials.

MATERIALS
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Brand
Product Description

Sigma-Aldrich
Anti-Huntingtin (N-terminal) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution