Thioredoxin induces Tregs to generate an immunotolerant tumor microenvironment in metastatic melanoma.

Thioredoxin induces Tregs to generate an immunotolerant tumor microenvironment in metastatic melanoma.

Oncoimmunology (2015-09-26)

Xiaogang Wang, Haisheng Dong, Qi Li, Yingxian Li, An Hong

ABSTRACT

Metastatic melanoma is a highly aggressive cancer that is very difficult to treat. Additionally, the antitumor immune reaction of melanoma is still unclear. Here we demonstrate an association between the expression and secretion of the antioxidant protein thioredoxin (TRX) and increasing tumor stage and metastasis in melanoma. To elucidate the role of TRX in melanoma, we assessed the correlation of TRX expression with different disease parameters in melanoma. We also examined the

MATERIALS

Product Number

Brand

Product Description

18304

Sigma-Aldrich

Hydrogen peroxide solution, 34.5-36.5%

316989

Sigma-Aldrich

Hydrogen peroxide solution, contains potassium stannate as inhibitor, 30-32 wt. % in water, semiconductor grade, 99.999% trace metals basis

296309

Sigma-Aldrich

1,4-Dioxane, anhydrous, 99.8%, contains <=25 ppm BHT as stabilizer

H1009

Sigma-Aldrich

Hydrogen peroxide solution, 30 % (w/w) in H₂O, contains stabilizer

CDS020367

Sigma-Aldrich

9,10-Dihydroacridine, Aldrich^CPR