A peptide from human semenogelin I self-assembles into a pH-responsive hydrogel.

A peptide from human semenogelin I self-assembles into a pH-responsive hydrogel.

Soft matter (2014-11-20)

B Frohm, J E DeNizio, D S M Lee, L Gentile, U Olsson, J Malm, K S Akerfeldt, S Linse

ABSTRACT

The peptide GSFSIQYTYHV derived from human semenogelin I forms a transparent hydrogel through spontaneous self-assembly in water at neutral pH. Linear rheology measurements demonstrate that the gel shows a dominating elastic response over a large frequency interval. CD, fluorescence and FTIR spectroscopy and cryo-TEM studies imply long fibrillar aggregates of extended β-sheet. Dynamic light scattering data indicate that the fibril lengths are of the order of micrometers. Time-dependent thioflavin T fluorescence shows that fibril formation by GSFSIQYTYHV is a nucleated reaction. The peptide may serve as basis for development of smart biomaterials of low immunogenicity suitable for biomedical applications, including drug delivery and wound healing.

MATERIALS

Product Number

Brand

Product Description

43617

Supelco

Sodium hydroxide concentrate, 0.1 M NaOH in water (0.1N), Eluent concentrate for IC

75746

Sigma-Aldrich

Sodium dodecyl sulfate, ≥99.0% (GC), dust-free pellets

71687

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Sodium hydroxide, BioUltra, for luminescence, ≥98.0% (T), pellets

72068

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Sodium hydroxide solution, BioUltra, for molecular biology, 10 M in H₂O

71717

Sigma-Aldrich

Sodium dodecyl sulfate, tested according to NF, mixture of sodium alkyl sulfates consisting mainly of sodium dodecyl sulfate

72064

Supelco

Sodium hydroxide solution, 49-51% in water, eluent for IC

227056

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N,N-Dimethylformamide, anhydrous, 99.8%

71726

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Sodium dodecyl sulfate, suitable for ion pair chromatography, LiChropur^™, ≥99.0%

62862

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Sodium dodecyl sulfate, ≥90% ((Assay))

72438

Supelco

N,N-Dimethylformamide, analytical standard

71725

Sigma-Aldrich

Sodium dodecyl sulfate, BioUltra, for molecular biology, ≥99.0% (GC)

437573

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N,N-Dimethylformamide, ACS reagent, ≥99.8%

436143

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Sodium dodecyl sulfate, ACS reagent, ≥99.0%

71729

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Sodium dodecyl sulfate, ≥98.0% (GC)

L5750

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Sodium dodecyl sulfate, 92.5-100.5% based on total alkyl sulfate content basis

L6026

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Sodium dodecyl sulfate, BioXtra, ≥99.0% (GC)

74255

Supelco

Sodium dodecyl sulfate, dust-free pellets, suitable for electrophoresis, for molecular biology, ≥99.0% (GC)

648531

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N,N-Dimethylformamide, suitable for HPLC, ≥99.9%

S8045

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S2770

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M2128

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Thiazolyl Blue Tetrazolium Bromide, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)

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901915

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30620

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