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  • Isolation and structure elucidation of rebaudioside D2 from bioconversion reaction of rebaudioside A to rebaudioside D.

Isolation and structure elucidation of rebaudioside D2 from bioconversion reaction of rebaudioside A to rebaudioside D.

Natural product communications (2014-09-23)
Indra Prakash, Cynthia Bunders, Krishna P Devkota, Romila D Charan, Catherine Ramirez, Maunik Parikh, Avetik Markosyan
ABSTRACT

We report the isolation and complete structure of an isomer of rebaudioside D, known as rebaudioside D2. This novel steviol glycoside was isolated from a bioconversion reaction of rebaudioside A to rebaudioside D. Rebaudioside D2 possesses a relatively rare 1 --> 6 sugar linkage, which was discovered by extensive analysis of NMR (1H, 13C, COSY, HSQC-DEPT, HMBC, 1D TOCSY and NOESY) and mass spectral data.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Pyridine-d5, ≥99.5 atom % D
Sigma-Aldrich
Pyridine-d5, "100%", ≥99.96 atom % D
Sigma-Aldrich
Pyridine-d5, ≥99.5 atom % D, contains 0.03 % (v/v) TMS