Lectin coated MgO nanoparticle: its toxicity, antileishmanial activity, and macrophage activation.

Lectin coated MgO nanoparticle: its toxicity, antileishmanial activity, and macrophage activation.

Drug and chemical toxicology (2014-01-08)

Ali Jebali, Seyedhossein Hekmatimoghaddam, Bahram Kazemi, Azra Allaveisie, Alireza Masoudi, Karim Daliri, Najme Sedighi, Javad Ranjbari

PMID24393043

ABSTRACT

The purpose of this research was to evaluate toxicity of uncoated magnesium oxide nanoparticles (MgO NPs), MgO NPs coated with Peanut agglutinin (PNA) lectin, and PNA alone on the promastigotes of Leishmania major (L. major) and macrophages of BALB/c mice. On the other hand, antileishmanial property of uncoated MgO NPs, lectin coated MgO NPs, and PNA lectin alone was evaluated, and also macrophage activation was investigated after treatment with these materials by measurement of nitrite, H2O2, and some interleukins. This study showed that PNA lectin and lectin coated MgO NPs had approximately no toxicity on L. major and macrophages, but some toxic effects were observed for uncoated MgO NPs, especially at concentration of 500 µg/mL. Interestingly, lectin coated MgO NPs had the highest antileishmanial activity and macrophage activation, compared with uncoated MgO NPs and PNA lectin.

MATERIALS

Product Number

Brand

Product Description

316989

Sigma-Aldrich

Hydrogen peroxide solution, contains potassium stannate as inhibitor, 30-32 wt. % in water, semiconductor grade, 99.999% trace metals basis

18304

Sigma-Aldrich

Hydrogen peroxide solution, 34.5-36.5%

95321

Supelco

Hydrogen peroxide solution, ≥30%, for trace analysis

82762

Supelco

Methanol, analytical standard

95294

Sigma-Aldrich

Hydrogen peroxide solution, tested according to Ph. Eur.

46874

Supelco

Sulfanilamide, VETRANAL^®, analytical standard

88597

Millipore

Hydrogen peroxide solution, 3%, suitable for microbiology

W292907

Sigma-Aldrich

Isopropyl alcohol, ≥99.7%, FCC, FG

322415

Sigma-Aldrich

Methanol, anhydrous, 99.8%

H1009

Sigma-Aldrich

Hydrogen peroxide solution, 30 % (w/w) in H₂O, contains stabilizer

S9251

Sigma-Aldrich

Sulfanilamide, ≥98%

33626

Sigma-Aldrich

Sulfanilamide, puriss. p.a., ≥98% (calc. to the dried substance)

1633007

USP

Sulfanilamide Melting Point Standard, United States Pharmacopeia (USP) Reference Standard

PHR1093

Supelco

Sulfanilamide melting point standard, Pharmaceutical Secondary Standard; Certified Reference Material

16911

Supelco

Hydrogen peroxide solution, 30 % (w/w), for ultratrace analysis

717975

Sigma-Aldrich

Methanol, NMR reference standard

S2151000

Sulfanilamide, European Pharmacopoeia (EP) Reference Standard

650609

Sigma-Aldrich

Methanol, HPLC Plus, ≥99.9%, poly-coated bottles

PHR1372

Supelco

Methanol, Pharmaceutical Secondary Standard; Certified Reference Material

676780

Sigma-Aldrich

Methanol, ACS reagent, ≥99.8%

M1775

Sigma-Aldrich

Methanol, Absolute - Acetone free

154903

Sigma-Aldrich

Methanol, ACS spectrophotometric grade, ≥99.9%

216763

Sigma-Aldrich

Hydrogen peroxide solution, contains inhibitor, 30 wt. % in H₂O, ACS reagent

323381

Sigma-Aldrich

Hydrogen peroxide solution, contains ~200 ppm acetanilide as stabilizer, 3 wt. % in H₂O

320390

Sigma-Aldrich

Methanol, ACS reagent, ≥99.8%

24229

Sigma-Aldrich

Methanol, puriss., meets analytical specification of Ph Eur, ≥99.7% (GC)

516813

Sigma-Aldrich

Hydrogen peroxide solution, 50 wt. % in H₂O, stabilized

32213

Sigma-Aldrich

Methanol, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8% (GC)

31642

Sigma-Aldrich

Hydrogen Peroxide Solution, 30% (w/w), puriss. p.a., reag. ISO, reag. Ph. Eur.

349887

Sigma-Aldrich

Hydrogen peroxide solution, contains inhibitor, 35 wt. % in H₂O