Skip to Content
MilliporeSigma
  • Interleukin 17 regulates Mer tyrosine kinase-positive cells in Pseudomonas aeruginosa keratitis.

Interleukin 17 regulates Mer tyrosine kinase-positive cells in Pseudomonas aeruginosa keratitis.

Investigative ophthalmology & visual science (2014-10-10)
Cui Li, Sharon A McClellan, Ronald Barrett, Linda D Hazlett
ABSTRACT

To determine if IL-17 regulates Mer tyrosine kinase-positive (MerTK+) cells in Pseudomonas aeruginosa keratitis. Interleukin 17 was tested in normal and infected cornea of susceptible C57BL/6 and resistant BALB/c mice. The latter were treated with recombinant mouse (rm) IL-17; both groups were treated with IL-17 neutralizing antibody. Mice were infected, and clinical score, PCR, ELISA, and myeloperoxidase (MPO) assays tested expression of proinflammatory and anti-inflammatory mediators and polymorphonuclear neutrophilic leukocyte (PMN) infiltrate. Fas and Fas ligand (FasL) protein levels were assessed in both mouse strains, while MerTK+ cells were examined by immunostaining and cell sorting before and after IL-17 neutralization. The IL-17 mRNA and protein were higher in C57BL/6 versus BALB/c cornea after infection. The rmIL-17 treatment of BALB/c mice modified proinflammatory and anti-inflammatory mediators, but clinical score and MPO assay revealed no differences. However, only BALB/c mice treated with IL-17 neutralizing antibody showed increased disease, macrophage inflammatory protein (MIP) 2, and MPO levels. Fas and FasL protein levels, elevated earlier in BALB/c versus C57BL/6 mice, correlated with significantly more MerTK+ cells in BALB/c cornea at 3 days after infection. Neutralization of IL-17 in C57BL/6 mice elevated MerTK+ cells, while similar treatment of BALB/c mice significantly decreased them. These data provide evidence that IL-17 expression is higher in C57BL/6 versus BALB/c cornea after infection and that the latter group has more MerTK+ cells. Exogenous rmIL-17 failed to shift the disease response in resistant mice, but its neutralization resulted in worsened disease and reduced MerTK+ cells. Neutralization of IL-17 in C57BL/6 mice increased MerTK+ cells but did not dramatically shift the disease response.

MATERIALS
Product Number
Brand
Product Description

Supelco
DL-Dithiothreitol solution, 1 M in H2O
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
DL-Dithiothreitol solution, BioUltra, for molecular biology, ~1 M in H2O
SKU
Pack Size
Availability
Price
Quantity
USP
Cetrimonium bromide, United States Pharmacopeia (USP) Reference Standard
SKU
Pack Size
Availability
Price
Quantity
Fluorescein, European Pharmacopoeia (EP) Reference Standard
SKU
Pack Size
Availability
Price
Quantity
SAFC
Hexadecyltrimethylammonium bromide, USP/NF
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Fluorescein, for fluorescence, free acid
SKU
Pack Size
Availability
Price
Quantity
Supelco
Hexadecyltrimethylammonium bromide, analytical standard
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Hexadecyltrimethylammonium bromide, ≥96.0% (AT)
SKU
Pack Size
Availability
Price
Quantity
Supelco
Hexadecyltrimethylammonium bromide, suitable for ion pair chromatography, LiChropur
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Hexadecyltrimethylammonium bromide, for molecular biology, ≥99%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
β-D-Allose, rare aldohexose sugar
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Hexadecyltrimethylammonium bromide, BioXtra, ≥99%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Hexadecyltrimethylammonium bromide, BioUltra, for molecular biology, ≥99.0% (AT)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Hexadecyltrimethylammonium bromide, ≥98%
SKU
Pack Size
Availability
Price
Quantity