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  • Development and validation of spectrophotometric methods for determination of ceftazidime in pharmaceutical dosage forms.

Development and validation of spectrophotometric methods for determination of ceftazidime in pharmaceutical dosage forms.

Acta pharmaceutica (Zagreb, Croatia) (2008-12-24)
Basavaraj Hiremath, Bennikallu Hire Mathada Mruthyunjayaswamy
ABSTRACT

Two spectrophotometric methods for the determination of ceftazidime (CFZM) in either pure form or in its pharmaceutical formulations are described. The first method is based on the reaction of 3-methylbenzothiazolin-2-one hydrazone (MBTH) with ceftazidime in the presence of ferric chloride in acidic medium. The resulting blue complex absorbs at lambdamax 628 nm. The second method describes the reaction between the diazotized drug and N-(1-naphthyl)ethylenediamine dihydrochloride (NEDA) to yield a purple colored product with lambdamax at 567 nm. The reaction conditions were optimized to obtain maximum color intensity. The absorbance was found to increase linearly with increasing the concentration of CFZM; the systems obeyed the Beer's law in the range 2-10 and 10-50 microg mL-1 for MBTH and NEDA methods, resp. LOD, LOQ and correlation coefficient values were 0.15, 0.79 and 0.50, 2.61. No interference was observed from common excipients present in pharmaceutical formulations. The proposed methods are simple, sensitive, accurate and suitable for quality control applications.

MATERIALS
Product Number
Brand
Product Description

Supelco
N-(1-Naphthyl)ethylenediamine dihydrochloride monomethanolate, for spectrophotometric det. of nitrate and nitrite, ≥99.0%
Sigma-Aldrich
N-(1-Naphthyl)ethylenediamine dihydrochloride, ACS reagent, >98%
Supelco
N-(1-Naphthyl)ethylenediamine dihydrochloride, for determination of sulfonamide and nitrite, ACS reagent, ≥98%
Sigma-Aldrich
N-(1-Naphthyl)ethylenediamine dihydrochloride, ≥98%
Sigma-Aldrich
Ammonium sulfamate, BioXtra, ≥98.0%