Skip to Content
MilliporeSigma
  • Enzyme-linked immunosorbent assay for total sennosides using anti-sennside A and anti-sennoside B monoclonal antibodies.

Enzyme-linked immunosorbent assay for total sennosides using anti-sennside A and anti-sennoside B monoclonal antibodies.

Fitoterapia (2008-10-18)
Osamu Morinaga, Takuhiro Uto, Seiichi Sakamoto, Hiroyuki Tanaka, Yukihiro Shoyama
ABSTRACT

Total sennosides concentration is a very important factor when rhubarb and senna will be used as crude drugs. However, one-step analytical technique for total sennosides has not been reported except HPLC. An enzyme-linked immunosorbent assay (ELISA) for total sennosides concentration by using the combination of anti-sennoside A (SA) and anti-sennoside B (SB) monoclonal antibodies (MAbs) in a single assay has been investigated. Total sennosides concentration in rhubarb and senna samples determined by newly developed assay system showed good agreement with those analyzed by ELISA using anti-SA MAb and anti-SB MAb, respectively.

MATERIALS
Product Number
Brand
Product Description

Supelco
Sennoside A, analytical standard
Sennoside B, primary reference standard