Glycoprotein labeling using engineered variants of galactose oxidase obtained by directed evolution.

Glycoprotein labeling using engineered variants of galactose oxidase obtained by directed evolution.

Journal of the American Chemical Society (2011-04-30)

Julie B Rannes, Avgousta Ioannou, Simon C Willies, Gideon Grogan, Carsten Behrens, Sabine L Flitsch, Nicholas J Turner

ABSTRACT

A directed evolution approach has been used for the generation of variants of galactose oxidase (GOase) that can selectively oxidize glycans on glycoproteins. The aldehyde function introduced on the glycans D-mannose (Man) and D-N-acetyl glucosamine (GlcNAc) by the enzyme variants could then be used to label the glycoproteins and also whole cells that display mannosides on their surface.

MATERIALS

Product Number

Brand

Product Description

G7400

Sigma-Aldrich

Galactose Oxidase from Dactylium dendroides, lyophilized powder, ≥3,000 units/g solid

G7907

Sigma-Aldrich

Galactose Oxidase from Dactylium dendroides, ≥30 units/mg solid